Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsxAB/MqsA(antitoxin)
Location 7353..7846 Replicon plasmid pC21-1
Accession NZ_CP052878
Organism Escherichia coli strain C21

Toxin (Protein)


Gene name TsxA Uniprot ID U9Y9W8
Locus tag HJD21_RS22700 Protein ID WP_001230709.1
Coordinates 7353..7607 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name TsxB Uniprot ID U9Y4R8
Locus tag HJD21_RS22705 Protein ID WP_000151456.1
Coordinates 7604..7846 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HJD21_RS22665 2928..3089 + 162 WP_001434039.1 type I toxin-antitoxin system Hok family toxin -
HJD21_RS22670 3238..3834 + 597 WP_000125165.1 hypothetical protein -
HJD21_RS24445 3852..4136 - 285 WP_001162371.1 hypothetical protein -
HJD21_RS22680 4210..4875 - 666 WP_015387337.1 P-loop NTPase -
HJD21_RS22685 5462..5791 + 330 WP_000855119.1 hypothetical protein -
HJD21_RS22690 6195..6710 + 516 WP_001025393.1 J domain-containing protein -
HJD21_RS22695 6814..7356 + 543 WP_001083904.1 hypothetical protein -
HJD21_RS22700 7353..7607 + 255 WP_001230709.1 hypothetical protein Toxin
HJD21_RS22705 7604..7846 + 243 WP_000151456.1 hypothetical protein Antitoxin
HJD21_RS22710 7983..8315 + 333 WP_001393253.1 WbuC family cupin fold metalloprotein -
HJD21_RS22715 8362..9237 - 876 WP_015387340.1 class A extended-spectrum beta-lactamase CTX-M-55 -
HJD21_RS22720 9490..9654 - 165 Protein_12 transposase -
HJD21_RS22725 9827..11026 - 1200 WP_000948429.1 IS91 family transposase -
HJD21_RS22730 11036..11224 - 189 WP_000957857.1 hypothetical protein -
HJD21_RS22735 11346..12440 - 1095 Protein_15 IS1380-like element ISEc9 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid blaCTX-M-55 - 1..63878 63878


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 9942.47 Da        Isoelectric Point: 10.1206

>T155719 WP_001230709.1 NZ_CP052878:7353-7607 [Escherichia coli]
MRKNKGRLTYYLEVIDKKYHFVKKISSYSKEFTDGKTKRTKRTLSELVFNESEVEAIDFTKNGLRPVDKNILLTMVKEYK
ESDA

Download         Length: 255 bp

>T155719 NZ_CP068812:4081911-4082309 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9136.45 Da        Isoelectric Point: 7.2775

>AT155719 WP_000151456.1 NZ_CP052878:7604-7846 [Escherichia coli]
MTIENTPENIKKLRKKIGLTQTECGEIFGVGLSTWQKKEAKTHNQLNLSKGEFEYLLLLAGEHPEYVLHKKAESRSPEEP

Download         Length: 243 bp

>AT155719 NZ_CP068812:4081615-4081908 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAATCCAGCAAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAACTGGTGAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3G9H8F0

Antitoxin

Source ID Structure
AlphaFold DB A0A3G9GNF5

References