155715

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 3512987..3513824 Replicon chromosome
Accession NZ_CP052877
Organism Escherichia coli strain C21

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag HJD21_RS16870 Protein ID WP_000227784.1
Coordinates 3513282..3513824 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag HJD21_RS16865 Protein ID WP_001297137.1
Coordinates 3512987..3513298 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HJD21_RS16840 3508007..3508954 + 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -
HJD21_RS16845 3508976..3510967 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
HJD21_RS16850 3510957..3511571 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
HJD21_RS16855 3511571..3511900 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
HJD21_RS16860 3511912..3512802 + 891 WP_000971336.1 heme o synthase -
HJD21_RS16865 3512987..3513298 + 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
HJD21_RS16870 3513282..3513824 + 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
HJD21_RS16875 3513880..3514815 - 936 WP_001297127.1 sel1 repeat family protein -
HJD21_RS16880 3515223..3516587 + 1365 WP_001000978.1 MFS transporter -
HJD21_RS16885 3516715..3517206 - 492 WP_001138904.1 nucleotide binding protein YajQ -
HJD21_RS16890 3517374..3518285 + 912 WP_000705874.1 2-dehydropantoate 2-reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T155715 WP_000227784.1 NZ_CP052877:3513282-3513824 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T155715 NZ_CP068812:3557988-3558338 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGTGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGGTGAGGTGGTGGAAGAGGC
GTTATTACGTTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT155715 WP_001297137.1 NZ_CP052877:3512987-3513298 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT155715 NZ_CP068812:3557743-3557994 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCACGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References