Detailed information of TA system TA155712

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2415043..2415681
• Replicon: chromosome
• Accession: NZ_CP052877
• Organism: Escherichia coli strain C21

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: HJD21_RS11640
• Protein ID: WP_000813794.1
• Coordinates: 2415505..2415681 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: HJD21_RS11635
• Protein ID: WP_001270285.1
• Coordinates: 2415043..2415459 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HJD21_RS11615	2410195..2411136	-	942	WP_001251313.1	ABC transporter permease	-
HJD21_RS11620	2411137..2412150	-	1014	WP_000220411.1	ABC transporter ATP-binding protein	-
HJD21_RS11625	2412168..2413313	-	1146	WP_000034363.1	ABC transporter substrate-binding protein	-
HJD21_RS11630	2413558..2414964	-	1407	WP_000760589.1	PLP-dependent aminotransferase family protein	-
HJD21_RS11635	2415043..2415459	-	417	WP_001270285.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
HJD21_RS11640	2415505..2415681	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
HJD21_RS11645	2415903..2416133	+	231	WP_000494244.1	YncJ family protein	-
HJD21_RS11650	2416225..2418186	-	1962	WP_001307191.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
HJD21_RS11655	2418259..2418795	-	537	WP_000429153.1	DNA-binding transcriptional regulator SutR	-
HJD21_RS11660	2418848..2420062	+	1215	WP_001326689.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2420102..2421250	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (88-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T155712 WP_000813794.1 NZ_CP052877:c2415681-2415505 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T155712 NZ_CP068812:3099525-3099836 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15247.61 Da
• Isoelectric Point: 4.6115

>AT155712 WP_001270285.1 NZ_CP052877:c2415459-2415043 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFDLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT155712 NZ_CP068812:3099837-3100127 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACACCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGAAC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACTGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAGGCACTTAACCGTTAG

Structures

Toxin

• PDB: 6HPB