Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 262242..262873 Replicon chromosome
Accession NZ_CP052877
Organism Escherichia coli strain C21

Toxin (Protein)


Gene name hicA Uniprot ID F4NNK6
Locus tag HJD21_RS01160 Protein ID WP_001259388.1
Coordinates 262242..262517 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag HJD21_RS01165 Protein ID WP_000593555.1
Coordinates 262514..262873 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HJD21_RS01145 257246..258313 + 1068 WP_000361482.1 HlyD family efflux transporter periplasmic adaptor subunit -
HJD21_RS01150 258310..261045 + 2736 WP_000149132.1 ribosome-associated ATPase/putative transporter RbbA -
HJD21_RS01155 261045..262169 + 1125 WP_001615305.1 ABC transporter permease -
HJD21_RS01160 262242..262517 + 276 WP_001259388.1 type II toxin-antitoxin system HicA family toxin Toxin
HJD21_RS01165 262514..262873 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
HJD21_RS01170 262993..263394 - 402 WP_001190064.1 nickel-responsive transcriptional regulator NikR -
HJD21_RS01175 263400..264206 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
HJD21_RS01180 264203..264967 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
HJD21_RS01185 264967..265800 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
HJD21_RS01190 265797..266741 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10151.89 Da        Isoelectric Point: 10.7076

>T155695 WP_001259388.1 NZ_CP052877:262242-262517 [Escherichia coli]
MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T155695 NZ_CP068812:c122057-121863 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT155695 WP_000593555.1 NZ_CP052877:262514-262873 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT155695 NZ_CP068812:121686-121864 [Escherichia coli]
GAGGACTGAAGTCTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGGCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FFA2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References