Detailed information of TA system TA155694

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 161182..161825
• Replicon: plasmid p3846III
• Accession: NZ_CP052873
• Organism: Enterobacter cloacae strain 3849

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: HJI43_RS24700
• Protein ID: WP_103793600.1
• Coordinates: 161409..161825 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A345CHX5
• Locus tag: HJI43_RS24695
• Protein ID: WP_039032651.1
• Coordinates: 161182..161412 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HJI43_RS24670	156589..157722	-	1134	WP_103793597.1	glutathione-independent formaldehyde dehydrogenase	-
HJI43_RS24675	157794..158063	+	270	WP_103793598.1	hypothetical protein	-
HJI43_RS24680	158182..158604	-	423	WP_095463659.1	GNAT family N-acetyltransferase	-
HJI43_RS24685	159183..159923	+	741	WP_022652289.1	hypothetical protein	-
HJI43_RS24690	160293..160682	+	390	WP_158251945.1	TonB family protein	-
HJI43_RS24695	161182..161412	+	231	WP_039032651.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HJI43_RS24700	161409..161825	+	417	WP_103793600.1	type II toxin-antitoxin system VapC family toxin	Toxin
HJI43_RS24705	162014..162340	+	327	WP_103793601.1	four-helix bundle copper-binding protein	-
HJI43_RS24925	162706..162798	-	93	Protein_169	DUF4113 domain-containing protein	-
HJI43_RS24710	163635..163820	+	186	WP_103793602.1	general stress protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..190697	190697

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-50)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15045.42 Da
• Isoelectric Point: 6.4523

>T155694 WP_103793600.1 NZ_CP052873:161409-161825 [Enterobacter cloacae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRNQRIVISAITYSEMRFGATGPKASPRHIQLVDAFCARLDAILPWDSA
AVDATTDIKVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVN

Nucleotide

• Length: 417 bp

>T155694 NZ_CP068811:c5007749-5007438 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8737.83 Da
• Isoelectric Point: 4.3654

>AT155694 WP_039032651.1 NZ_CP052873:161182-161412 [Enterobacter cloacae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQLEKADPDFMAEREDVVTDEGRFDL

Nucleotide

• Length: 231 bp

>AT155694 NZ_CP068811:c5007437-5007147 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACACCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGAAC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACTGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAGGCACTTAACCGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A345CHX5