Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 61882..62408 Replicon plasmid p3846_IncHI2_mcr
Accession NZ_CP052871
Organism Enterobacter cloacae strain 3849

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag HJI43_RS22245 Protein ID WP_000323025.1
Coordinates 61882..62169 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag HJI43_RS22250 Protein ID WP_000534858.1
Coordinates 62169..62408 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HJI43_RS22210 57452..57628 - 177 WP_001371930.1 hypothetical protein -
HJI43_RS22215 58139..59083 + 945 WP_000778029.1 DUF5417 domain-containing protein -
HJI43_RS22220 59182..59781 + 600 WP_001031420.1 hypothetical protein -
HJI43_RS22225 59841..60191 + 351 WP_000743059.1 hypothetical protein -
HJI43_RS22230 60238..60441 + 204 WP_001015183.1 hypothetical protein -
HJI43_RS22235 60723..61043 + 321 WP_000332796.1 hypothetical protein -
HJI43_RS22240 61652..61810 - 159 WP_001447866.1 type I toxin-antitoxin system Hok family toxin -
HJI43_RS22245 61882..62169 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
HJI43_RS22250 62169..62408 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
HJI43_RS22255 62671..63594 - 924 WP_000167917.1 cation diffusion facilitator family transporter -
HJI43_RS22260 63794..64366 - 573 WP_001515348.1 cytochrome b/b6 domain-containing protein -
HJI43_RS22265 64842..66080 - 1239 WP_000219087.1 IS110 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid ant(2'')-Ia / aadA2 / qacE / sul1 / blaSHV-12 / tet(A) / qnrA1 / dfrA16 / blaCTX-M-9 / mcr-9 - 1..293138 293138


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T155690 WP_000323025.1 NZ_CP052871:c62169-61882 [Enterobacter cloacae]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T155690 NZ_CP068811:c4520610-4520320 [Escherichia coli]
ATGAACTATGAGCTGGCGTTTGATCCGCGGGCGCTGAAAGAGTGGCAAAAACTGGGCGTGACAGTCCGGGAACAATTTAA
AAAAAAGCTTGAGGATGTGCTGAAAAGGCCGCGCAATCCATCGGCTAAACTGCGGGATTTACCGGACTGCTACAAAATTA
AGCTGCGCACACAGGGATATCGCCTGGTTTATCAGGTTAACGATAAAGAGTTGCTCGTGTTGGTGATAGCTATTGGTAAG
AGAGAGAACTCAGCCGTTTATGAAGACGCGACTAAACGGCTGGATGAGTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT155690 WP_000534858.1 NZ_CP052871:c62408-62169 [Enterobacter cloacae]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT155690 NZ_CP068811:c4520842-4520600 [Escherichia coli]
ATGGCCACACTTAACGTCCGCCTGGATGACAAACTGAAAAATGAGGCTTATGCGGTGCTGGAAAAATTAAATATCACGCC
TACTGAAGCAGTGCGGCTGCTGTTTCAGTACGTTGCGGAAAATGGACGAATGCCGGTAAAAACGCTCACTGTCAGTGATA
GTGAAGAAGCCTTGTTACGCACTGTCAGGGAACGACTGGCCAACCCACAAGAGGCCATTAAGGTCCGACTGGATGAACTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References