Detailed information of TA system TA155674

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapC-vagC/VapC-VagC
• Location: 104351..104988
• Replicon: plasmid pBGHN1-1
• Accession: NZ_CP052865
• Organism: Burkholderia glumae strain HN1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: C5AP38
• Locus tag: HJC54_RS12270
• Protein ID: WP_012735347.1
• Coordinates: 104587..104988 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: HJC54_RS12265
• Protein ID: WP_017924531.1
• Coordinates: 104351..104587 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HJC54_RS12250	101134..102636	+	1503	Protein_97	IS21 family transposase	-
HJC54_RS12255	102676..103425	+	750	WP_169511691.1	IS21-like element helper ATPase IstB	-
HJC54_RS12260	103555..104214	+	660	WP_169511692.1	tyrosine-type recombinase/integrase	-
HJC54_RS12265	104351..104587	+	237	WP_017924531.1	AbrB family transcriptional regulator	Antitoxin
HJC54_RS12270	104587..104988	+	402	WP_012735347.1	type II toxin-antitoxin system VapC family toxin	Toxin
HJC54_RS12275	105237..105566	+	330	WP_155297075.1	hypothetical protein	-
HJC54_RS12280	105584..105826	+	243	WP_039205039.1	hypothetical protein	-
HJC54_RS12285	105967..106695	+	729	WP_012735342.1	IS6 family transposase	-
HJC54_RS12290	106894..107016	-	123	Protein_105	AAA family ATPase	-
HJC54_RS12295	107101..107742	-	642	WP_169511693.1	DsbA family protein	-
HJC54_RS12300	107766..108677	-	912	WP_161522130.1	SDR family NAD(P)-dependent oxidoreductase	-
HJC54_RS12305	108707..109360	-	654	WP_161522129.1	SDR family oxidoreductase	-
HJC54_RS12310	109365..109982	-	618	WP_169511694.1	NAD(P)H-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	fliL	1..185112	185112
	-	flank	IS/Tn	-	-	102676..103425	749

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-117)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14847.12 Da
• Isoelectric Point: 5.2138

>T155674 WP_012735347.1 NZ_CP052865:104587-104988 [Burkholderia glumae]
MPRFMLDTNMCIYLMKNQPEQVAKRFAECFVGDVVMSAITYAELEYGVAVSDNRAKERRNLAALIEDILVAPFDAAAAVA
YGPIREATRERKKDHLDKLIAAHAVALDVVLVTNNAKDFASYPGIKLDNWLND

Nucleotide

• Length: 402 bp

>T155674 NZ_CP068811:1219913-1220224 [Escherichia coli]
ATGCACATCATTTCGAAAGCGCCATTTGAAGAGTGTGCGCGCAAATATCCTAACGACGCTCTGGCTCTACACTCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCTAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8965.10 Da
• Isoelectric Point: 6.3676

>AT155674 WP_017924531.1 NZ_CP052865:104351-104587 [Burkholderia glumae]
MQTTRVFRNGNSQAVRIPADLAYERSDVELEIERVGDEIRIRPARRPLTGVLKKFAKFSQDFMAEGRGEQEQAEREGL

Nucleotide

• Length: 237 bp

>AT155674 NZ_CP068811:1220221-1220640 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCAGCAATGCCGGTT
GGCGTGGCCTTACTTCGTACGCTGATTGATCAACATAATTTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
TCTGGTCAGTCAGATTTTGTCTGGGCAACGATCCCTGACGATATCCCATATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: C5AP38