Detailed information of TA system TA155673

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 2282721..2283496
• Replicon: chromosome
• Accession: NZ_CP052864
• Organism: Burkholderia glumae strain HN1

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: HJC54_RS09800
• Protein ID: WP_017423245.1
• Coordinates: 2282999..2283496 (+)
• Length: 166 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: -
• Locus tag: HJC54_RS09795
• Protein ID: WP_161521884.1
• Coordinates: 2282721..2283002 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HJC54_RS09790	2282028..2282465	+	438	WP_169511094.1	SMI1/KNR4 family protein	-
HJC54_RS09795	2282721..2283002	+	282	WP_161521884.1	DUF1778 domain-containing protein	Antitoxin
HJC54_RS09800	2282999..2283496	+	498	WP_017423245.1	GNAT family N-acetyltransferase	Toxin
HJC54_RS09805	2283577..2284059	+	483	WP_161521885.1	ProQ activator of osmoprotectant transporter prop	-
HJC54_RS09810	2284416..2285351	+	936	Protein_1947	RHS domain-containing protein	-
HJC54_RS09815	2285368..2285652	+	285	WP_153478924.1	hypothetical protein	-
HJC54_RS09820	2285775..2286320	+	546	Protein_1949	IS3 family transposase	-
HJC54_RS09825	2286573..2287922	-	1350	WP_015877376.1	allantoin permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2258599..2293995	35396
	-	flank	IS/Tn	-	-	2285802..2286344	542

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (44-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (5-91)

Sequences

Toxin

Protein

• Length: 166 a.a.
• Molecular weight: 17606.19 Da
• Isoelectric Point: 9.6894

>T155673 WP_017423245.1 NZ_CP052864:2282999-2283496 [Burkholderia glumae]
MTGWRAPEPLSDAHGLDDFDSGVASLDTWLKRRALANQRSGASRTFVATRDGRVGAYYALASGAVTLEVAPGRFRRNMPD
PIPVVVLGRLAVDRAHQGIGVGRALVRDAGLRVLHAAAAIGIRSLIVHALSDSAKAFYERVGFEASPLDPMLLLITLADL
EHALS

Nucleotide

• Length: 498 bp

>T155673 NZ_CP068811:1122335-1122670 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACGGTTGCCCCAGAGGAATTACAGCTCATTAAAGCCAAAATTAA
CGTGCTGATTGGGTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10226.50 Da
• Isoelectric Point: 5.0477

>AT155673 WP_161521884.1 NZ_CP052864:2282721-2283002 [Burkholderia glumae]
MASTTPRDTLNLRIRPDDRSLIDRAAAAAHKTRTDFVLDAARAAAEQTLLDQVVLSAEPQAYAAFLARLDEPVQPNEALR
QTMQTRAPWDPAA

Nucleotide

• Length: 282 bp

>AT155673 NZ_CP068811:1122087-1122335 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA