Detailed information of TA system TA155669

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 4583354..4584981
• Replicon: chromosome
• Accession: NZ_CP052862
• Organism: Pseudomonas sp. ADAK2

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: HKK52_RS20945
• Protein ID: WP_169372409.1
• Coordinates: 4583354..4584706 (+)
• Length: 451 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: HKK52_RS20950
• Protein ID: WP_169372410.1
• Coordinates: 4584703..4584981 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HKK52_RS20940	4580199..4583156	-	2958	WP_169372408.1	autotransporter domain-containing protein	-
HKK52_RS20945	4583354..4584706	+	1353	WP_169372409.1	HipA domain-containing protein	Toxin
HKK52_RS20950	4584703..4584981	+	279	WP_169372410.1	helix-turn-helix domain-containing protein	Antitoxin
HKK52_RS20955	4585072..4585908	-	837	WP_169372411.1	amino acid ABC transporter permease	-
HKK52_RS20960	4585905..4586726	-	822	WP_169372412.1	ABC transporter substrate-binding protein	-
HKK52_RS20965	4586838..4587737	+	900	WP_169372413.1	homocysteine S-methyltransferase family protein	-
HKK52_RS20970	4587846..4588517	-	672	WP_169372414.1	GNAT family N-acetyltransferase	-
HKK52_RS20975	4588535..4588981	-	447	WP_169372415.1	GNAT family N-acetyltransferase	-
HKK52_RS20980	4588991..4589614	-	624	WP_169372416.1	FMN-binding negative transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (165-367)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-82)

Sequences

Toxin

Protein

• Length: 451 a.a.
• Molecular weight: 50466.88 Da
• Isoelectric Point: 4.7507

>T155669 WP_169372409.1 NZ_CP052862:4583354-4584706 [Pseudomonas sp. ADAK2]
MYNLTLQVYTAGNWHDAMVLSFDNPEKGFESRCSFGYEQSYLVDNYADIPTPFAKAVSARFPLEWDGKRSNAPAFLHDIA
PAGAAKRFLQQRIGREKPAGISEDLFLLSTSTPAPIGNMRIKESVDVVGQREVIGFKRQDVISRDNRFLEYAYEQGAAIG
GATGAGGEAPKLLLAQDKDGDLYPDAVLDDADVSQHWFVKFSRNKGTQNDQDILRSEYHYYKALQTLGIETVAVEGLALE
EATKPSLWMQRFDRRVTPQGVERFAVESIYSLAQVTTPGSPMNHMEVIRLLAELWRDGAQENQIPDLVADYLRRDLINKI
LGNSDNHGRNTAIIRDTASFRMAPIYDLAPMVMDEEGVTRTTKWPKELERAGDVNWRGVCQALADIADPDDPLAALSDWS
GSFERLREDATRLMALPDILADSGLPDVTMNHPRIDLKNLARRLKEWDLI

Nucleotide

• Length: 1353 bp

>T155669 NZ_CP068811:264429-264536 [Escherichia coli]
ATGACGCTCGCAGAACTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CATGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10339.11 Da
• Isoelectric Point: 10.1890

>AT155669 WP_169372410.1 NZ_CP052862:4584703-4584981 [Pseudomonas sp. ADAK2]
MSMTVAERTVLIESIQEALAEGTLEIGEAIRRLRVEVTGLHQTQFAKMCKISVRTLVHIEHGEGNQTLKSLNSVFKPFGL
KMGVVRIRRDFS

Nucleotide

• Length: 279 bp

>AT155669 NZ_CP068811:c264380-264317 [Escherichia coli]
GTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT