Detailed information of TA system TA155663

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 4583359..4584986
• Replicon: chromosome
• Accession: NZ_CP052861
• Organism: Pseudomonas sp. ADAK7

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: HKK53_RS20950
• Protein ID: WP_169372409.1
• Coordinates: 4583359..4584711 (+)
• Length: 451 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: HKK53_RS20955
• Protein ID: WP_169372410.1
• Coordinates: 4584708..4584986 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HKK53_RS20945	4580204..4583161	-	2958	WP_169372408.1	autotransporter domain-containing protein	-
HKK53_RS20950	4583359..4584711	+	1353	WP_169372409.1	HipA domain-containing protein	Toxin
HKK53_RS20955	4584708..4584986	+	279	WP_169372410.1	helix-turn-helix domain-containing protein	Antitoxin
HKK53_RS20960	4585077..4585913	-	837	WP_169372411.1	amino acid ABC transporter permease	-
HKK53_RS20965	4585910..4586731	-	822	WP_169372412.1	ABC transporter substrate-binding protein	-
HKK53_RS20970	4586843..4587742	+	900	WP_169372413.1	homocysteine S-methyltransferase family protein	-
HKK53_RS20975	4587851..4588522	-	672	WP_169372414.1	GNAT family N-acetyltransferase	-
HKK53_RS20980	4588540..4588986	-	447	WP_169372415.1	GNAT family N-acetyltransferase	-
HKK53_RS20985	4588996..4589619	-	624	WP_169372416.1	FMN-binding negative transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (165-367)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-82)

Sequences

Toxin

Protein

• Length: 451 a.a.
• Molecular weight: 50466.88 Da
• Isoelectric Point: 4.7507

>T155663 WP_169372409.1 NZ_CP052861:4583359-4584711 [Pseudomonas sp. ADAK7]
MYNLTLQVYTAGNWHDAMVLSFDNPEKGFESRCSFGYEQSYLVDNYADIPTPFAKAVSARFPLEWDGKRSNAPAFLHDIA
PAGAAKRFLQQRIGREKPAGISEDLFLLSTSTPAPIGNMRIKESVDVVGQREVIGFKRQDVISRDNRFLEYAYEQGAAIG
GATGAGGEAPKLLLAQDKDGDLYPDAVLDDADVSQHWFVKFSRNKGTQNDQDILRSEYHYYKALQTLGIETVAVEGLALE
EATKPSLWMQRFDRRVTPQGVERFAVESIYSLAQVTTPGSPMNHMEVIRLLAELWRDGAQENQIPDLVADYLRRDLINKI
LGNSDNHGRNTAIIRDTASFRMAPIYDLAPMVMDEEGVTRTTKWPKELERAGDVNWRGVCQALADIADPDDPLAALSDWS
GSFERLREDATRLMALPDILADSGLPDVTMNHPRIDLKNLARRLKEWDLI

Nucleotide

• Length: 1353 bp

>T155663 NZ_CP068810:c4780798-4780625 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10339.11 Da
• Isoelectric Point: 10.1890

>AT155663 WP_169372410.1 NZ_CP052861:4584708-4584986 [Pseudomonas sp. ADAK7]
MSMTVAERTVLIESIQEALAEGTLEIGEAIRRLRVEVTGLHQTQFAKMCKISVRTLVHIEHGEGNQTLKSLNSVFKPFGL
KMGVVRIRRDFS

Nucleotide

• Length: 279 bp

>AT155663 NZ_CP068810:c4781122-4780826 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCATTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG