Detailed information of TA system TA155660

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 2878900..2879524
• Replicon: chromosome
• Accession: NZ_CP052861
• Organism: Pseudomonas sp. ADAK7

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: HKK53_RS13400
• Protein ID: WP_169371212.1
• Coordinates: 2878900..2879079 (+)
• Length: 60 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: HKK53_RS13405
• Protein ID: WP_169371213.1
• Coordinates: 2879117..2879524 (+)
• Length: 136 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HKK53_RS13375	2874726..2876213	+	1488	WP_169371208.1	sensor domain-containing diguanylate cyclase	-
HKK53_RS13380	2876206..2876424	+	219	WP_169371209.1	cysteine-rich CWC family protein	-
HKK53_RS13385	2876459..2877151	+	693	WP_169371210.1	pseudouridine synthase	-
HKK53_RS13390	2877464..2878348	+	885	WP_169371211.1	alpha/beta hydrolase	-
HKK53_RS13400	2878900..2879079	+	180	WP_169371212.1	type II toxin-antitoxin system HicA family toxin	Toxin
HKK53_RS13405	2879117..2879524	+	408	WP_169371213.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
HKK53_RS13410	2880002..2880265	+	264	WP_123507545.1	short-chain dehydrogenase	-
HKK53_RS13415	2880645..2881373	+	729	WP_169371214.1	class I SAM-dependent methyltransferase	-
HKK53_RS13420	2881454..2882224	-	771	WP_169371215.1	trans-aconitate 2-methyltransferase	-
HKK53_RS13425	2882619..2883122	+	504	WP_169371216.1	inclusion body family protein	-
HKK53_RS13430	2883246..2883785	+	540	WP_169371217.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-128)

Sequences

Toxin

Protein

• Length: 60 a.a.
• Molecular weight: 6723.00 Da
• Isoelectric Point: 11.4113

>T155660 WP_169371212.1 NZ_CP052861:2878900-2879079 [Pseudomonas sp. ADAK7]
VNSRFLISQIVADGWYLVRIRGSHHHFKHPTKPGLVTVPHPKKDLLKKTAMSILQQALL

Nucleotide

• Length: 180 bp

>T155660 NZ_CP068810:4564676-4564933 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGCCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 136 a.a.
• Molecular weight: 14662.68 Da
• Isoelectric Point: 4.4904

>AT155660 WP_169371213.1 NZ_CP052861:2879117-2879524 [Pseudomonas sp. ADAK7]
MLYPISISMGDDEHAWGVEVPDIPGCFSAGEDLDDAMAMAREAIEGHFEILAEDGSPIPPANKVTLHAANPKYAGCTWAL
VDIDVTKYLGKAQKLNITLPGYLLNRIDEYVLHHPEEKSRSGFLASAALKVLQQG

Nucleotide

• Length: 408 bp

>AT155660 NZ_CP068810:4564945-4565496 [Escherichia coli]
ATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCGACCTTCAAAGGAGAAATGGATTCACCGCTGATGCAC
ATCCTCGCTCCCCTGGCGGTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCGCAGGCCCTGATGAAACCGGAGCACTGGCGTGAATAA