Detailed information of TA system TA155657

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 3710938..3711554
• Replicon: chromosome
• Accession: NZ_CP052860
• Organism: Pseudomonas sp. ADAK13

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: HKK54_RS17115
• Protein ID: WP_169387298.1
• Coordinates: 3711372..3711554 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A7Z2XSN8
• Locus tag: HKK54_RS17110
• Protein ID: WP_010176619.1
• Coordinates: 3710938..3711348 (-)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HKK54_RS17075	3706251..3706739	-	489	WP_169387293.1	DMT family transporter	-
HKK54_RS17080	3706808..3707731	+	924	WP_169387294.1	LysR family transcriptional regulator	-
HKK54_RS17085	3707759..3708163	+	405	WP_169387295.1	fosfomycin resistance glutathione transferase	-
HKK54_RS17090	3708340..3708867	+	528	WP_003212082.1	DUF4142 domain-containing protein	-
HKK54_RS17095	3708923..3709336	-	414	WP_010176622.1	low affinity iron permease family protein	-
HKK54_RS17100	3709516..3710298	+	783	WP_169387296.1	GNAT family N-acetyltransferase	-
HKK54_RS17105	3710299..3710805	-	507	WP_169387297.1	methylated-DNA--[protein]-cysteine S-methyltransferase	-
HKK54_RS17110	3710938..3711348	-	411	WP_010176619.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
HKK54_RS17115	3711372..3711554	-	183	WP_169387298.1	type II toxin-antitoxin system HicA family toxin	Toxin
HKK54_RS17120	3711725..3712633	-	909	WP_169387299.1	LysR family transcriptional regulator	-
HKK54_RS17125	3712729..3713892	+	1164	WP_169387300.1	MFS transporter	-
HKK54_RS17130	3713889..3715082	+	1194	WP_169387301.1	MFS transporter	-
HKK54_RS17135	3715193..3716005	-	813	WP_169387302.1	helix-turn-helix transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (12-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-128)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6764.93 Da
• Isoelectric Point: 10.7532

>T155657 WP_169387298.1 NZ_CP052860:c3711554-3711372 [Pseudomonas sp. ADAK13]
VDSRYLIGHNVADGWYLVRIRGSHHPFKHPTKPGLVTIPHPKKDLLDKTAKSILKQALLS

Nucleotide

• Length: 183 bp

>T155657 NZ_CP068810:c4087250-4086330 [Escherichia coli]
ATGGATCTGCGTGATAGCTATGCCCGGCAGGTCAAACTGTTGATGGCCGCTCTGCCGTATGTGGCGAAGGAGTCCTGTTT
CGCGTTGAAAGGCGGCACCGCAATCAATTTGTTTGTGCAGGATTTTCCCCGTCTGTCGGTGGATATTGATCTGGCGTACA
AGAGCTTTGCGGATCGCGATACTGATTTGGCGGCTATCAACGACGCTTTGAAGAGGATTACTGCATCGCTGAACGGCAGA
CCGGGGATCACCGCGATTCGGCAGGAAAATAAAGCGGATGAAAAGCGCATCATTGTGAATACCGCTGATGCAAAGATTAA
GATCGAGGTTAGCCCGGTCTGGCGCGGGCTGTTGTTGCCTCCGGCAGAAATGCCTGTGTGCGAACGTGTGGAAATGGAGT
ATGGCTTCACCACCATGAGTGTCGTCTCGCTGGCGGATCTTTATGGCGGTAAAATCTGCGCTGCGCTTGATCGTCAGCAC
CCGCGCGATCTGTTTGATGTGCTGAATATGCTGGAGAAACCGGGACTGATGCGGGAGATCTTTGACGGTTTTCTTTGTTA
TCTGGCAGGCCATCCCCGCCCCATCGCCGAGTTGCTGGCTCCAAACTGGGATACAGAGCGGATCGCCACGTTGTACGCGC
AGGAATTTTCAGGGATGACGCAACAGGAAACGTCGCTGGAATCGCTTCTGTCAGTAACGACTTTGCTGCCGCAGGCGTTG
AAGTCACATCTTACGGATCTGGATCGCGAGTTCTTGCTCAGTTTTAAGCAAAACAGCCCTGACTGGTCGCGGTATCGCTA
TCCTGAAATTCAGCATCTTCCGGCTATTCGCTGGAAGCAACGCAATCTGGCGGTGCTTAAAGATAAAAATCCGGCGAAGT
ATGTGGCGGCGGTGAATAAGCTAGAGAGGGTTCTGGCGTAA

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 14788.74 Da
• Isoelectric Point: 4.4077

>AT155657 WP_010176619.1 NZ_CP052860:c3711348-3710938 [Pseudomonas sp. ADAK13]
MLYPIAISTGDEDHAWGVEVPDIPGCFSAGEDLDDAMAMAREAIEGHFEILAEDGAPIPAAQKVTLHAANPQYAGCTWAV
VDIDVTKYLGKAQKLNITLPGYLLNRIDEYVLHHPEEKSRSGFLASAALKVLQQDR

Nucleotide

• Length: 411 bp

>AT155657 NZ_CP068810:c4088019-4087237 [Escherichia coli]
TTGCCAAACTATCTAAACTGGCTGATGCAGCACACGCTACCCGGCCAGGTGCTGCTCCAGTCATGGCTTACCCGCAACGG
CATAGATCGTAAGCTCTCTTATCTCTATGTGAAGAATGGCTGGCTCAGGCGGATCGCTCACGGCGTATACTGCCGGACGG
GTCGTGAACCGGATTGGGTGGATGCGGTTTACTGCCTGCAATCGCAGTGGGAAAAGCCTGTTCGGGTGGCGGGGCTAACC
AGCCTTGCGCTTCAGGGTCATGCGCACTATACCGAACAGGGTAAGCCGCAGGTCTGGCTCGCGCTTCCGGCTTACGTCAA
ACTGCCGGGATGGTTTTCTGCCTTTGAACAATCCGCAACGTTTGTCACGCTGTATACGTCAGGCCTTACCGCTGATGTAA
CGCCATTTACTACGGAACTGAAAATTGGCGACCGCCAGTTAGCAGGCAGTGTTCCGGAACTGGCCGCGTATGAGATCGCC
TGCGGCGTTCCTTCGTTAATCTCGTTCGAACATGCCGATGCTCTGTTTCAGGGGTTGAATCTTCTCAGCCCACGCAAATT
GCAGGCACTGCTTCGCGCCAGCCATTCGGTTAAAACCAACCGGGTGATGCTTTACCTTGCCCGGCGTAATGGACACCCTT
ATTTTCAGTATATTGATCAAAGCGGTATAGAAACAGGTACGGGAAAGCGGCAAATCATACCCGGCGGCTGGCTGGAGCCG
GATTATCAAATTACTGTTCCACGCGCATTTAAACCGGAAGGAGTCGAAGATGGATCTGCGTGA

Structures

Antitoxin

• AlphaFold DB: A0A7Z2XSN8