Detailed information of TA system TA155647

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 5427384..5428933
• Replicon: chromosome
• Accession: NZ_CP052858
• Organism: Pseudomonas sp. ADAK20

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: HKK56_RS24130
• Protein ID: WP_169391185.1
• Coordinates: 5427384..5428658 (+)
• Length: 425 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A1S1UPJ9
• Locus tag: HKK56_RS24135
• Protein ID: WP_029290025.1
• Coordinates: 5428655..5428933 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HKK56_RS24110	5422472..5423887	+	1416	WP_029290035.1	acetyl-CoA carboxylase biotin carboxylase subunit	-
HKK56_RS24115	5423899..5425707	+	1809	WP_029290033.1	sodium-extruding oxaloacetate decarboxylase subunit alpha	-
HKK56_RS24120	5425774..5427042	-	1269	WP_169390041.1	autotransporter outer membrane beta-barrel domain-containing protein	-
HKK56_RS24125	5427231..5427485	-	255	WP_169389581.1	hypothetical protein	-
HKK56_RS24130	5427384..5428658	+	1275	WP_169391185.1	HipA domain-containing protein	Toxin
HKK56_RS24135	5428655..5428933	+	279	WP_029290025.1	helix-turn-helix domain-containing protein	Antitoxin
HKK56_RS24140	5428937..5429767	-	831	WP_029290023.1	amino acid ABC transporter permease	-
HKK56_RS24145	5429764..5430585	-	822	WP_154907136.1	ABC transporter substrate-binding protein	-
HKK56_RS24150	5430699..5431598	+	900	WP_169390040.1	homocysteine S-methyltransferase family protein	-
HKK56_RS24155	5431700..5432371	-	672	WP_070996666.1	GNAT family N-acetyltransferase	-
HKK56_RS24160	5432408..5432854	-	447	WP_070996668.1	GNAT family N-acetyltransferase	-
HKK56_RS24165	5432841..5433488	-	648	WP_029290014.1	FMN-binding negative transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-351)

Antitoxin

• Cro/C1-type helix-turn-helix domain (27-80)

Sequences

Toxin

Protein

• Length: 425 a.a.
• Molecular weight: 47006.35 Da
• Isoelectric Point: 5.5772

>T155647 WP_169391185.1 NZ_CP052858:5427384-5428658 [Pseudomonas sp. ADAK20]
MRLEFSEPQNGFDGPCRFHYRQDYLVEHLDDIEQGFCSAVSGRFPLEWDVTFLKTAPAFLHDIAPAGAAKRFLLQRMGAD
KPDDVSVDLFLLGRCTPSPIGNLRIKESVEALGVRPAVGFSRHDVVSRDNGFLEYAYEQGAAIGGATGAGGEAPKLLMAE
HRDGLLYPDAVLPDAEVAQHWFIKFARNGATLNDQAILRSEFHYYKALQALGIETVAAQGLALEEAHKPSLWMQRFDRNV
TSAGVERLAVESIYSLAGVVTPGSYLDHLHVVQMLAEHWRQIGQGDGVADLVAEYLRRDLLNKILGNSDNHGRNTSIIRE
RNSMRLAPIYDLAPMVMDAEGITRTTKWPKGLEVAGEIDWQGVCGALEGLVDPQQAFERLRADAEHLRALPDILVNSGLP
QETWKHPAIALGKLDQRLQQWGLR

Nucleotide

• Length: 1275 bp

>T155647 NZ_CP068810:1191556-1191891 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACGGTTGCCCCAGAGGAATTACAGCTCATTAAAGCCAAAATTAA
CGTGCTGATTGGGTAG

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10197.95 Da
• Isoelectric Point: 11.9342

>AT155647 WP_029290025.1 NZ_CP052858:5428655-5428933 [Pseudomonas sp. ADAK20]
MTVSMDTRALLIQGIEQGLAQGTLQIGEAVRRLRVEVTGLHQSQFARMCKISVRTLVHIEHGEGNQTLKSLNAVFRPFGL
QMGVVRVRRSLG

Nucleotide

• Length: 279 bp

>AT155647 NZ_CP068810:1191308-1191556 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A1S1UPJ9