Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 698638..699254 Replicon chromosome
Accession NZ_CP052858
Organism Pseudomonas sp. ADAK20

Toxin (Protein)


Gene name hicA Uniprot ID A0A7Y1DDS6
Locus tag HKK56_RS03175 Protein ID WP_029299101.1
Coordinates 699072..699254 (-) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A1S1UT55
Locus tag HKK56_RS03170 Protein ID WP_070995801.1
Coordinates 698638..699048 (-) Length 137 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HKK56_RS03140 694890..695378 - 489 WP_029299107.1 DMT family transporter -
HKK56_RS03145 695447..696373 + 927 WP_070995800.1 LysR family transcriptional regulator -
HKK56_RS03150 696439..697080 + 642 WP_029299105.1 LysE family translocator -
HKK56_RS03155 697199..697726 + 528 WP_029299104.1 DUF4142 domain-containing protein -
HKK56_RS03160 697883..698029 + 147 WP_166647832.1 hypothetical protein -
HKK56_RS03165 698035..698535 - 501 WP_029299103.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
HKK56_RS03170 698638..699048 - 411 WP_070995801.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
HKK56_RS03175 699072..699254 - 183 WP_029299101.1 type II toxin-antitoxin system HicA family toxin Toxin
HKK56_RS03185 699741..700625 - 885 WP_169389771.1 alpha/beta hydrolase -
HKK56_RS03190 700925..701617 - 693 WP_133714153.1 pseudouridine synthase -
HKK56_RS03195 701617..701829 - 213 WP_169389770.1 cysteine-rich CWC family protein -
HKK56_RS03200 702048..702407 + 360 WP_029299096.1 hypothetical protein -
HKK56_RS03205 702550..702978 + 429 WP_029299095.1 DUF3757 domain-containing protein -
HKK56_RS03210 703001..704113 - 1113 WP_029299094.1 enoyl-CoA hydratase/isomerase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(6-57)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-128)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6773.94 Da        Isoelectric Point: 10.8600

>T155643 WP_029299101.1 NZ_CP052858:c699254-699072 [Pseudomonas sp. ADAK20]
VDSRYLIGHIVADGWYLARVRGSHHHFKHPTKPGLVTIPHPRKELLDKTAKSILKQALLG

Download         Length: 183 bp

>T155643 NZ_CP068810:737477-737773 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCACTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCTGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin


Download         Length: 137 a.a.        Molecular weight: 14811.77 Da        Isoelectric Point: 4.5025

>AT155643 WP_070995801.1 NZ_CP052858:c699048-698638 [Pseudomonas sp. ADAK20]
MLYPIAISTGDDDHAWGVEVPDIPGCFSAGDDLDDAMAMAREAIEGHFELLAEDGAPIPSAQKVTHHAANPLYAGCIWAV
VDIDVTKYLGKAQKLNITLPGYLLNRIDEYVLHHPEEKSRSGFLASAALKVLQQER

Download         Length: 411 bp

>AT155643 NZ_CP068810:737775-738170 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7Y1DDS6

Antitoxin

Source ID Structure
AlphaFold DB A0A1S1UT55

References