Detailed information of TA system TA155640

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 1943151..1944700
• Replicon: chromosome
• Accession: NZ_CP052857
• Organism: Pseudomonas sp. ADAK21

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: HKK57_RS08955
• Protein ID: WP_169391185.1
• Coordinates: 1943426..1944700 (-)
• Length: 425 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A1S1UPJ9
• Locus tag: HKK57_RS08950
• Protein ID: WP_029290025.1
• Coordinates: 1943151..1943429 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HKK57_RS08920	1938596..1939243	+	648	WP_029290014.1	FMN-binding negative transcriptional regulator	-
HKK57_RS08925	1939230..1939676	+	447	WP_070996668.1	GNAT family N-acetyltransferase	-
HKK57_RS08930	1939713..1940384	+	672	WP_070996666.1	GNAT family N-acetyltransferase	-
HKK57_RS08935	1940486..1941385	-	900	WP_169390040.1	homocysteine S-methyltransferase family protein	-
HKK57_RS08940	1941499..1942320	+	822	WP_154907136.1	ABC transporter substrate-binding protein	-
HKK57_RS08945	1942317..1943147	+	831	WP_029290023.1	amino acid ABC transporter permease	-
HKK57_RS08950	1943151..1943429	-	279	WP_029290025.1	helix-turn-helix domain-containing protein	Antitoxin
HKK57_RS08955	1943426..1944700	-	1275	WP_169391185.1	HipA domain-containing protein	Toxin
HKK57_RS08960	1944599..1944853	+	255	WP_169389581.1	hypothetical protein	-
HKK57_RS08965	1945042..1946310	+	1269	WP_169390041.1	autotransporter outer membrane beta-barrel domain-containing protein	-
HKK57_RS08970	1946377..1948185	-	1809	WP_029290033.1	sodium-extruding oxaloacetate decarboxylase subunit alpha	-
HKK57_RS08975	1948197..1949612	-	1416	WP_029290035.1	acetyl-CoA carboxylase biotin carboxylase subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-351)

Antitoxin

• Cro/C1-type helix-turn-helix domain (27-80)

Sequences

Toxin

Protein

• Length: 425 a.a.
• Molecular weight: 47006.35 Da
• Isoelectric Point: 5.5772

>T155640 WP_169391185.1 NZ_CP052857:c1944700-1943426 [Pseudomonas sp. ADAK21]
MRLEFSEPQNGFDGPCRFHYRQDYLVEHLDDIEQGFCSAVSGRFPLEWDVTFLKTAPAFLHDIAPAGAAKRFLLQRMGAD
KPDDVSVDLFLLGRCTPSPIGNLRIKESVEALGVRPAVGFSRHDVVSRDNGFLEYAYEQGAAIGGATGAGGEAPKLLMAE
HRDGLLYPDAVLPDAEVAQHWFIKFARNGATLNDQAILRSEFHYYKALQALGIETVAAQGLALEEAHKPSLWMQRFDRNV
TSAGVERLAVESIYSLAGVVTPGSYLDHLHVVQMLAEHWRQIGQGDGVADLVAEYLRRDLLNKILGNSDNHGRNTSIIRE
RNSMRLAPIYDLAPMVMDAEGITRTTKWPKGLEVAGEIDWQGVCGALEGLVDPQQAFERLRADAEHLRALPDILVNSGLP
QETWKHPAIALGKLDQRLQQWGLR

Nucleotide

• Length: 1275 bp

>T155640 NZ_CP068810:217181-217288 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTCTGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10197.95 Da
• Isoelectric Point: 11.9342

>AT155640 WP_029290025.1 NZ_CP052857:c1943429-1943151 [Pseudomonas sp. ADAK21]
MTVSMDTRALLIQGIEQGLAQGTLQIGEAVRRLRVEVTGLHQSQFARMCKISVRTLVHIEHGEGNQTLKSLNAVFRPFGL
QMGVVRVRRSLG

Nucleotide

• Length: 279 bp

>AT155640 NZ_CP068810:c217132-217069 [Escherichia coli]
GTCTAGATGCAAGAATGGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Structures

Antitoxin

• AlphaFold DB: A0A1S1UPJ9