Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 668914..669530 Replicon chromosome
Accession NZ_CP052857
Organism Pseudomonas sp. ADAK21

Toxin (Protein)


Gene name hicA Uniprot ID A0A7Y1DDS6
Locus tag HKK57_RS03115 Protein ID WP_029299101.1
Coordinates 668914..669096 (+) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A1S1UT55
Locus tag HKK57_RS03120 Protein ID WP_070995801.1
Coordinates 669120..669530 (+) Length 137 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HKK57_RS03080 664055..665167 + 1113 WP_029299094.1 enoyl-CoA hydratase/isomerase family protein -
HKK57_RS03085 665190..665618 - 429 WP_029299095.1 DUF3757 domain-containing protein -
HKK57_RS03090 665761..666120 - 360 WP_029299096.1 hypothetical protein -
HKK57_RS03095 666339..666551 + 213 WP_169389770.1 cysteine-rich CWC family protein -
HKK57_RS03100 666551..667243 + 693 WP_133714153.1 pseudouridine synthase -
HKK57_RS03105 667543..668427 + 885 WP_169389771.1 alpha/beta hydrolase -
HKK57_RS03115 668914..669096 + 183 WP_029299101.1 type II toxin-antitoxin system HicA family toxin Toxin
HKK57_RS03120 669120..669530 + 411 WP_070995801.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
HKK57_RS03125 669633..670133 + 501 WP_029299103.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
HKK57_RS03130 670139..670285 - 147 WP_166647832.1 hypothetical protein -
HKK57_RS03135 670442..670969 - 528 WP_029299104.1 DUF4142 domain-containing protein -
HKK57_RS03140 671088..671729 - 642 WP_029299105.1 LysE family translocator -
HKK57_RS03145 671795..672721 - 927 WP_070995800.1 LysR family transcriptional regulator -
HKK57_RS03150 672790..673278 + 489 WP_029299107.1 DMT family transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(6-57)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-128)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6773.94 Da        Isoelectric Point: 10.8600

>T155638 WP_029299101.1 NZ_CP052857:668914-669096 [Pseudomonas sp. ADAK21]
VDSRYLIGHIVADGWYLARVRGSHHHFKHPTKPGLVTIPHPRKELLDKTAKSILKQALLG

Download         Length: 183 bp

>T155638 NZ_CP068810:161180-161365 [Escherichia coli]
GTGAAAAGTGCAGACGTGATCGCCATCCTGAAGCAGCATGGCTGGGAACATATTCGAACCCGTGGCAGTCATCATCAGTT
CCGCCACCCTGTTCATCCGGGTCTGGTGACGGTTCCGCACCCTAAAAAAGATATTAAGCCCGGTACGCTGGCGCAAATTG
GGCGTCAGGCTGGTTTAACATTTTAA

Antitoxin


Download         Length: 137 a.a.        Molecular weight: 14811.77 Da        Isoelectric Point: 4.5025

>AT155638 WP_070995801.1 NZ_CP052857:669120-669530 [Pseudomonas sp. ADAK21]
MLYPIAISTGDDDHAWGVEVPDIPGCFSAGDDLDDAMAMAREAIEGHFELLAEDGAPIPSAQKVTHHAANPLYAGCIWAV
VDIDVTKYLGKAQKLNITLPGYLLNRIDEYVLHHPEEKSRSGFLASAALKVLQQER

Download         Length: 411 bp

>AT155638 NZ_CP068810:161382-161792 [Escherichia coli]
ATGTTTTATCCCGCCTATATCCACAGTGATCCTGACGGCAGCGCCAGCGGTTTTTTCCCTGATGTTCCAGGCTGTTATTT
TGCTGGTGATACGCTGGACAACGCTTTCCAGGATGCGCGAAGTGCACTGGTCGCGCACTTCGAAACCTTATGCGAGATGA
ATGAAGAGTTGCCGCTTCCAGGCTCTGTGGAAACACATCTGGTCCAGCGTGCGCAGGACTTTATTGGTGGGCAATGGTTG
CTGGTGGATATCAATATGAATCAGTTCGAAGGTCGGGCTGAACGTATCAATATTACGATGCCGAAAAGGTTACTGAACAA
GATTGATACCTACGTGCGCAATAACCCAGACTACGCCAACAGAAGTGCGTTTCTGGCAGAAGCTGCCCGGCGTGTATTGC
CGGGCGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7Y1DDS6

Antitoxin

Source ID Structure
AlphaFold DB A0A1S1UT55

References