Detailed information of TA system TA155630

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 677241..677833
• Replicon: chromosome
• Accession: NZ_CP052855
• Organism: Xylella fastidiosa subsp. multiplex strain Fillmore

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A060HCK8
• Locus tag: HKJ31_RS02970
• Protein ID: WP_004085590.1
• Coordinates: 677241..677537 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: HKJ31_RS02975
• Protein ID: WP_004085592.1
• Coordinates: 677540..677833 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HKJ31_RS02940	672247..672507	-	261	WP_212590801.1	hypothetical protein	-
HKJ31_RS02945	672525..673502	-	978	WP_169710315.1	peptidase	-
HKJ31_RS02950	674041..674268	+	228	WP_169710316.1	hypothetical protein	-
HKJ31_RS02955	674269..675432	-	1164	WP_169710168.1	phage tail protein	-
HKJ31_RS02960	675436..675996	-	561	WP_004084996.1	DUF2612 domain-containing protein	-
HKJ31_RS02965	675993..677138	-	1146	WP_169710318.1	baseplate J/gp47 family protein	-
HKJ31_RS02970	677241..677537	+	297	WP_004085590.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HKJ31_RS02975	677540..677833	+	294	WP_004085592.1	putative addiction module antidote protein	Antitoxin
HKJ31_RS02980	677889..678242	-	354	WP_169710320.1	hypothetical protein	-
HKJ31_RS02985	678239..678880	-	642	WP_012382583.1	hypothetical protein	-
HKJ31_RS02990	678877..679707	-	831	WP_004085594.1	hypothetical protein	-
HKJ31_RS02995	679704..680021	-	318	WP_169710077.1	hypothetical protein	-
HKJ31_RS03000	680021..680791	-	771	WP_169710321.1	hypothetical protein	-
HKJ31_RS03005	680839..681129	+	291	WP_004087089.1	hypothetical protein	-
HKJ31_RS03010	681113..681379	+	267	WP_004087087.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	672247..718420	46173

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-94)

Antitoxin

• Uncharacterized protein HI1420 (3-93)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11169.70 Da
• Isoelectric Point: 10.0610

>T155630 WP_004085590.1 NZ_CP052855:677241-677537 [Xylella fastidiosa subsp. multiplex]
MIELKQTDTFRKWREKLKDARARSAIASRLDRLAFGHVGDAEPVGKGVSELRINYGPGYRVYFQRRGDTIYLLLCGGDKG
SQARDIKTALHLSEQWSE

Nucleotide

• Length: 297 bp

>T155630 NZ_CP068809:c3842604-3842227 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGAGGCATCGCGCTGCCTGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCACTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AAGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCCCTGGTGCGTACCGACCAGCCGGGATTC
AGCCCCTGTACCCGCTCTCAGTTAATCAACAGTATCGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACCCGCGA
TAACTACAGAACGGTAAATAACATTACCCGGGGTAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10246.59 Da
• Isoelectric Point: 5.6232

>AT155630 WP_004085592.1 NZ_CP052855:677540-677833 [Xylella fastidiosa subsp. multiplex]
MTEKLTSYDPAEDLTTDQAIADFMAAAFGTNDPAYVAHALGVVARAKGMTQIASQTGLSREQLYRSFSAEGNPTLRTTLA
VMKALGIELSAKPSGVH

Nucleotide

• Length: 294 bp

>AT155630 NZ_CP068809:c3843062-3842694 [Escherichia coli]
GTGTCAGACGCACTCTCCGGGACAATGCTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACAGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCTGACACTCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A060HCK8