Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 513541..514127 Replicon chromosome
Accession NZ_CP052855
Organism Xylella fastidiosa subsp. multiplex strain Fillmore

Toxin (Protein)


Gene name hicA Uniprot ID Q87BV5
Locus tag HKJ31_RS02220 Protein ID WP_004089517.1
Coordinates 513873..514127 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID B2I6C9
Locus tag HKJ31_RS02215 Protein ID WP_004085025.1
Coordinates 513541..513876 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HKJ31_RS02185 510315..510890 - 576 WP_004086888.1 terminase small subunit -
HKJ31_RS02190 511021..511245 - 225 WP_169710301.1 hypothetical protein -
HKJ31_RS02195 511247..511708 - 462 WP_169709972.1 hypothetical protein -
HKJ31_RS02200 511698..512027 - 330 WP_004085022.1 phage holin family protein -
HKJ31_RS02205 512020..512520 - 501 WP_169710303.1 lysozyme -
HKJ31_RS02210 512620..513351 - 732 WP_004085024.1 site-specific DNA-methyltransferase -
HKJ31_RS02215 513541..513876 - 336 WP_004085025.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
HKJ31_RS02220 513873..514127 - 255 WP_004089517.1 type II toxin-antitoxin system HicA family toxin Toxin
HKJ31_RS02225 514180..514902 - 723 WP_004085026.1 hypothetical protein -
HKJ31_RS02230 514845..515192 - 348 WP_162010044.1 hypothetical protein -
HKJ31_RS11305 516049..516648 + 600 WP_162008660.1 hypothetical protein -
HKJ31_RS02240 516645..516923 + 279 WP_169710305.1 VRR-NUC domain-containing protein -
HKJ31_RS02245 516925..517329 - 405 WP_004085029.1 hypothetical protein -
HKJ31_RS02250 517414..518832 + 1419 WP_169710307.1 DEAD/DEAH box helicase -
HKJ31_RS02255 518829..518993 + 165 WP_004086189.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 473940..521671 47731


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(23-80)

Antitoxin

Uncharacterised protein family HicB

(55-105)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 9639.14 Da        Isoelectric Point: 11.2766

>T155629 WP_004089517.1 NZ_CP052855:c514127-513873 [Xylella fastidiosa subsp. multiplex]
MKRKHAKTLSAIYTRPVSANIQWRDIEALFVELGAKVEEREGSRMLVRLFGERRIFHRPHPSPNTDKGAVESIRAWLKSN
GVTP

Download         Length: 255 bp

>T155629 NZ_CP068809:3652568-3652786 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGTCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCTGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12324.07 Da        Isoelectric Point: 5.6556

>AT155629 WP_004085025.1 NZ_CP052855:c513876-513541 [Xylella fastidiosa subsp. multiplex]
MKNIMTIESFKAIITYDPEIDMFRGEFVGINGGADFYAKDLKGLRREGAISLKVFLEACKEDSVEPRKCYSGKFNARISP
ELHALASEAAAAQGISLNQFVEQAIQHEVHT

Download         Length: 336 bp

>AT155629 NZ_CP068809:3652168-3652542 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q87BV5

Antitoxin

Source ID Structure
AlphaFold DB A0A060HAK5

References