Detailed information of TA system TA155628

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-dinJ/YafQ-RelB
• Location: 234708..235239
• Replicon: chromosome
• Accession: NZ_CP052855
• Organism: Xylella fastidiosa subsp. multiplex strain Fillmore

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: HKJ31_RS00985
• Protein ID: WP_004086966.1
• Coordinates: 234708..234989 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: A0A060H9I0
• Locus tag: HKJ31_RS00990
• Protein ID: WP_004086967.1
• Coordinates: 234976..235239 (-)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HKJ31_RS00955	230577..232450	+	1874	Protein_179	phage major capsid protein	-
HKJ31_RS00960	232468..232728	+	261	WP_004085160.1	hypothetical protein	-
HKJ31_RS00965	232728..233051	+	324	WP_154128257.1	hypothetical protein	-
HKJ31_RS00970	233048..233569	+	522	WP_169710272.1	hypothetical protein	-
HKJ31_RS00975	233545..234087	+	543	WP_169710274.1	hypothetical protein	-
HKJ31_RS00980	234084..234671	+	588	WP_169710276.1	phage baseplate assembly protein V	-
HKJ31_RS00985	234708..234989	-	282	WP_004086966.1	type II toxin-antitoxin system YafQ family toxin	Toxin
HKJ31_RS00990	234976..235239	-	264	WP_004086967.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
HKJ31_RS00995	235389..235727	+	339	WP_004085176.1	GPW/gp25 family protein	-
HKJ31_RS01000	235727..236620	+	894	WP_154128227.1	baseplate J/gp47 family protein	-
HKJ31_RS01005	236613..237170	+	558	WP_004086970.1	phage tail protein I	-
HKJ31_RS01010	237178..238770	+	1593	WP_169710278.1	tail fiber domain-containing protein	-
HKJ31_RS01015	238835..240013	+	1179	WP_169710148.1	phage tail sheath family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	197148..248148	51000

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (1-91)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (4-70)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10848.31 Da
• Isoelectric Point: 7.5505

>T155628 WP_004086966.1 NZ_CP052855:c234989-234708 [Xylella fastidiosa subsp. multiplex]
MRRISQTGQFKRDYKREAKGQHRATLDEELIHVLEALACDHPLEPRHHDHALTGGWKDHRDCHIKPDLVLIYRKPDNETL
QLVRIGSHSELGL

Nucleotide

• Length: 282 bp

>T155628 NZ_CP068809:c3623459-3623157 [Escherichia coli]
ATGGCTCAAAAGAAAAATATAAGGAGTTTTCGTGATGCCTGGTTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATTTTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9525.98 Da
• Isoelectric Point: 5.6033

>AT155628 WP_004086967.1 NZ_CP052855:c235239-234976 [Xylella fastidiosa subsp. multiplex]
MSANAVVRARVDTHIKEEASTVLATMGLSVSDAFRMMLTRIAREKALPFEPLVPNETTIAAMREARAGHLKSFDSIEALM
ADLHAED

Nucleotide

• Length: 264 bp

>AT155628 NZ_CP068809:c3623121-3622780 [Escherichia coli]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACCACTG
AGATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGTCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Structures

Antitoxin

• AlphaFold DB: A0A060H9I0