Detailed information of TA system TA155624

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 1302867..1303459
• Replicon: chromosome
• Accession: NZ_CP052854
• Organism: Xylella fastidiosa subsp. multiplex strain LM10

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A060HCK8
• Locus tag: HKJ32_RS05880
• Protein ID: WP_004085590.1
• Coordinates: 1303163..1303459 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: HKJ32_RS05875
• Protein ID: WP_004085592.1
• Coordinates: 1302867..1303160 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HKJ32_RS05845	1299289..1299549	+	261	WP_004085596.1	Blp family class II bacteriocin	-
HKJ32_RS05850	1299909..1300679	+	771	WP_169710165.1	hypothetical protein	-
HKJ32_RS05855	1300679..1300996	+	318	WP_012382585.1	hypothetical protein	-
HKJ32_RS05860	1300993..1301823	+	831	WP_004085594.1	hypothetical protein	-
HKJ32_RS05865	1301820..1302461	+	642	WP_169710166.1	hypothetical protein	-
HKJ32_RS05870	1302458..1302811	+	354	WP_004084998.1	hypothetical protein	-
HKJ32_RS05875	1302867..1303160	-	294	WP_004085592.1	putative addiction module antidote protein	Antitoxin
HKJ32_RS05880	1303163..1303459	-	297	WP_004085590.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HKJ32_RS05885	1303562..1304707	+	1146	WP_169710167.1	baseplate J/gp47 family protein	-
HKJ32_RS05890	1304704..1305264	+	561	WP_004084996.1	DUF2612 domain-containing protein	-
HKJ32_RS05895	1305268..1306431	+	1164	WP_169710168.1	phage tail protein	-
HKJ32_RS05900	1306461..1306940	-	480	WP_169710169.1	hypothetical protein	-
HKJ32_RS05905	1306974..1308089	-	1116	WP_038231256.1	HigA family addiction module antidote protein	-
HKJ32_RS05910	1308098..1308397	-	300	WP_004085585.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1257168..1336354	79186

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-94)

Antitoxin

• Uncharacterized protein HI1420 (3-93)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11169.70 Da
• Isoelectric Point: 10.0610

>T155624 WP_004085590.1 NZ_CP052854:c1303459-1303163 [Xylella fastidiosa subsp. multiplex]
MIELKQTDTFRKWREKLKDARARSAIASRLDRLAFGHVGDAEPVGKGVSELRINYGPGYRVYFQRRGDTIYLLLCGGDKG
SQARDIKTALHLSEQWSE

Nucleotide

• Length: 297 bp

>T155624 NZ_CP068809:2557774-2558052 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ATCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10246.59 Da
• Isoelectric Point: 5.6232

>AT155624 WP_004085592.1 NZ_CP052854:c1303160-1302867 [Xylella fastidiosa subsp. multiplex]
MTEKLTSYDPAEDLTTDQAIADFMAAAFGTNDPAYVAHALGVVARAKGMTQIASQTGLSREQLYRSFSAEGNPTLRTTLA
VMKALGIELSAKPSGVH

Nucleotide

• Length: 294 bp

>AT155624 NZ_CP068809:2558052-2558336 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCAGAA
AAAACAGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Structures

Toxin

• AlphaFold DB: A0A060HCK8