Detailed information of TA system TA155623

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): FicTA/Fic(toxin)
• Location: 1276038..1276834
• Replicon: chromosome
• Accession: NZ_CP052854
• Organism: Xylella fastidiosa subsp. multiplex strain LM10

Toxin (Protein)

• Gene name: VbhT
• Uniprot ID: -
• Locus tag: HKJ32_RS05680
• Protein ID: WP_004087040.1
• Coordinates: 1276223..1276834 (+)
• Length: 204 a.a.

Antitoxin (Protein)

• Gene name: VbhA
• Uniprot ID: A0A060HF63
• Locus tag: HKJ32_RS05675
• Protein ID: WP_004087046.1
• Coordinates: 1276038..1276226 (+)
• Length: 63 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HKJ32_RS05630	1271475..1272296	-	822	WP_004086371.1	DUF2303 family protein	-
HKJ32_RS05635	1272318..1272680	-	363	WP_169709949.1	hypothetical protein	-
HKJ32_RS05640	1272701..1272892	-	192	WP_169709948.1	hypothetical protein	-
HKJ32_RS05645	1273011..1273538	-	528	WP_004086367.1	hypothetical protein	-
HKJ32_RS05650	1273582..1273773	-	192	WP_004086365.1	hypothetical protein	-
HKJ32_RS05655	1273770..1274177	-	408	WP_154128431.1	hypothetical protein	-
HKJ32_RS05660	1274174..1274644	-	471	WP_027700621.1	DUF1566 domain-containing protein	-
HKJ32_RS05665	1274641..1275078	-	438	WP_038231390.1	hypothetical protein	-
HKJ32_RS05670	1275189..1275644	-	456	WP_004086362.1	hypothetical protein	-
HKJ32_RS05675	1276038..1276226	+	189	WP_004087046.1	antitoxin VbhA family protein	Antitoxin
HKJ32_RS05680	1276223..1276834	+	612	WP_004087040.1	Fic/DOC family protein	Toxin
HKJ32_RS05685	1276885..1277559	-	675	WP_169710212.1	helix-turn-helix transcriptional regulator	-
HKJ32_RS05690	1277644..1277889	+	246	WP_169710162.1	hypothetical protein	-
HKJ32_RS05695	1278148..1278339	+	192	WP_154128206.1	hypothetical protein	-
HKJ32_RS05700	1278367..1278555	+	189	WP_020851634.1	hypothetical protein	-
HKJ32_RS05705	1278552..1278800	+	249	WP_038231490.1	hypothetical protein	-
HKJ32_RS05710	1278797..1279180	+	384	WP_169710163.1	hypothetical protein	-
HKJ32_RS05715	1279383..1280495	+	1113	WP_004086911.1	Bro-N domain-containing protein	-
HKJ32_RS05720	1280616..1280906	+	291	WP_004086949.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1257168..1336354	79186

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (55-152)

Antitoxin

• Antitoxin VbhA (10-56)

Sequences

Toxin

Protein

• Length: 204 a.a.
• Molecular weight: 22961.76 Da
• Isoelectric Point: 5.1271

>T155623 WP_004087040.1 NZ_CP052854:1276223-1276834 [Xylella fastidiosa subsp. multiplex]
MKYAGDRGDPYLDSETGVLRNLLGIRDQGWLDKIESTLSFLRTSELRERPVKGKFDLAHLQEIHNRLFQDVYDWAGQIRQ
VEISKGNTMFAQQIAIQSAAQQIFGQLAKERFLCGLDAEEFSKRAGDYLGEINVLHPFREGNGRTQREFIAQLAQRAGYR
IDWGVVSQADMIKASIDAYNGDSSGLASIIREGISDQLFKDNE

Nucleotide

• Length: 612 bp

>T155623 NZ_CP068809:2511860-2513182 [Escherichia coli]
ATGCCTAAACTAGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCTGAGTGGTTAGCCAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCTAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGATAGCGTTGGTGCCGTGACGTTAATCCCCGAAGA
TGAAACCGTAACGTGTCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCGTTAGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCACAGGAGAAGACTGCGCTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAGGGAATAACGCCAACGACGCACATTATCAAATTGCCGATTGGCGAAAT
CAGACAGCCCAATGCGACGCTCGATCTTAGCCAAAGCGTTGATAATGAGTATTACTGTTTGCTGCTGGCGAAAGAACTCG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAGGGTGCGCGCGTTAGCGGTCAAACGTTTTGACAGGCGTTGG
AATACTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGCCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCCGGTATCGCGCAAATTATGGCTTTTTTAATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAGTTTCAGGTCTTCCAGTGGTTGATTGGCGCAACAGATGGCCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGGCTCACACCATTTTATGACATCATTTCGGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTCTGGCGACAGCAAAGGTGCTGAAATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCG
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAACCAATGTGTT
GAGGTTGCACGGTCGGTTAAGCCGAGAATACGGTATTAAGTAA

Antitoxin

Protein

• Length: 63 a.a.
• Molecular weight: 6855.80 Da
• Isoelectric Point: 4.9859

>AT155623 WP_004087046.1 NZ_CP052854:1276038-1276226 [Xylella fastidiosa subsp. multiplex]
MLTDHEKAERRAVVQSALASQRIEGLEPDAQAVVDAECWARGEMTIAAAVDQYKARVRLQMA

Nucleotide

• Length: 189 bp

>AT155623 NZ_CP068809:2511594-2511860 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTGGCGAATGCAATGAAACTGGTTCGCCAGCAAAACGGCTGGAC
GCAGAGCGAATTGGCGAAAAAAATCGGCATTAAGCAGGCGACAATTTCCAATTTCGAAAACAATCCTGACAATACCTCGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACGCGAAAAATGCCTCGCCAGAAGCA
GCAGAACAGCAAGATCTGGAGTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A060HF63