Detailed information of TA system TA155621

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-dinJ/YafQ-RelB
• Location: 944031..944562
• Replicon: chromosome
• Accession: NZ_CP052854
• Organism: Xylella fastidiosa subsp. multiplex strain LM10

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: HKJ32_RS04175
• Protein ID: WP_004086966.1
• Coordinates: 944281..944562 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: A0A060H9I0
• Locus tag: HKJ32_RS04170
• Protein ID: WP_004086967.1
• Coordinates: 944031..944294 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HKJ32_RS04145	939257..940435	-	1179	WP_169710148.1	phage tail sheath family protein	-
HKJ32_RS04150	940500..942092	-	1593	WP_154128230.1	tail fiber domain-containing protein	-
HKJ32_RS04155	942100..942657	-	558	WP_004086970.1	phage tail protein I	-
HKJ32_RS04160	942650..943543	-	894	WP_004085178.1	baseplate J/gp47 family protein	-
HKJ32_RS04165	943543..943881	-	339	WP_004088650.1	GPW/gp25 family protein	-
HKJ32_RS04170	944031..944294	+	264	WP_004086967.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
HKJ32_RS04175	944281..944562	+	282	WP_004086966.1	type II toxin-antitoxin system YafQ family toxin	Toxin
HKJ32_RS04180	944599..945186	-	588	WP_128283746.1	phage baseplate assembly protein V	-
HKJ32_RS04185	945183..945725	-	543	WP_154436958.1	hypothetical protein	-
HKJ32_RS04190	945701..946222	-	522	WP_154436960.1	hypothetical protein	-
HKJ32_RS04195	946219..946542	-	324	WP_154436962.1	hypothetical protein	-
HKJ32_RS04200	946542..946802	-	261	WP_012337650.1	hypothetical protein	-
HKJ32_RS04205	946820..948693	-	1874	Protein_817	phage major capsid protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	935639..952780	17141

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (1-91)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (4-70)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10848.31 Da
• Isoelectric Point: 7.5505

>T155621 WP_004086966.1 NZ_CP052854:944281-944562 [Xylella fastidiosa subsp. multiplex]
MRRISQTGQFKRDYKREAKGQHRATLDEELIHVLEALACDHPLEPRHHDHALTGGWKDHRDCHIKPDLVLIYRKPDNETL
QLVRIGSHSELGL

Nucleotide

• Length: 282 bp

>T155621 NZ_CP068809:c1940386-1940012 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTACGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9525.98 Da
• Isoelectric Point: 5.6033

>AT155621 WP_004086967.1 NZ_CP052854:944031-944294 [Xylella fastidiosa subsp. multiplex]
MSANAVVRARVDTHIKEEASTVLATMGLSVSDAFRMMLTRIAREKALPFEPLVPNETTIAAMREARAGHLKSFDSIEALM
ADLHAED

Nucleotide

• Length: 264 bp

>AT155621 NZ_CP068809:c1940843-1940475 [Escherichia coli]
GTGTCAGACACACTGCCCGGGACAACGCATCCCGACGACAATCACGACCGCCTCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGAAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGATGCAGATGCATACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAACGTAA

Structures

Antitoxin

• AlphaFold DB: A0A060H9I0