Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 514376..514962 Replicon chromosome
Accession NZ_CP052854
Organism Xylella fastidiosa subsp. multiplex strain LM10

Toxin (Protein)


Gene name hicA Uniprot ID Q87BV5
Locus tag HKJ32_RS02215 Protein ID WP_004089517.1
Coordinates 514708..514962 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID B2I6C9
Locus tag HKJ32_RS02210 Protein ID WP_004085025.1
Coordinates 514376..514711 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HKJ32_RS02180 511150..511725 - 576 WP_004086888.1 terminase small subunit -
HKJ32_RS02185 511856..512080 - 225 WP_012337656.1 hypothetical protein -
HKJ32_RS02190 512082..512543 - 462 WP_169709972.1 hypothetical protein -
HKJ32_RS02195 512533..512862 - 330 WP_004085022.1 phage holin family protein -
HKJ32_RS02200 512855..513355 - 501 WP_128382956.1 lysozyme -
HKJ32_RS02205 513455..514186 - 732 WP_169709925.1 site-specific DNA-methyltransferase -
HKJ32_RS02210 514376..514711 - 336 WP_004085025.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
HKJ32_RS02215 514708..514962 - 255 WP_004089517.1 type II toxin-antitoxin system HicA family toxin Toxin
HKJ32_RS02220 515015..515737 - 723 WP_012337661.1 hypothetical protein -
HKJ32_RS02230 516081..516239 + 159 WP_021358690.1 hypothetical protein -
HKJ32_RS12185 516884..517483 + 600 WP_162008660.1 hypothetical protein -
HKJ32_RS02240 517480..517758 + 279 WP_038230497.1 VRR-NUC domain-containing protein -
HKJ32_RS02245 517760..518164 - 405 WP_004085029.1 hypothetical protein -
HKJ32_RS02250 518249..519667 + 1419 WP_169709926.1 DEAD/DEAH box helicase -
HKJ32_RS02255 519664..519828 + 165 WP_004086189.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 474774..523587 48813


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(23-80)

Antitoxin

Uncharacterised protein family HicB

(55-105)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 9639.14 Da        Isoelectric Point: 11.2766

>T155620 WP_004089517.1 NZ_CP052854:c514962-514708 [Xylella fastidiosa subsp. multiplex]
MKRKHAKTLSAIYTRPVSANIQWRDIEALFVELGAKVEEREGSRMLVRLFGERRIFHRPHPSPNTDKGAVESIRAWLKSN
GVTP

Download         Length: 255 bp

>T155620 NZ_CP068809:1929937-1930191 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12324.07 Da        Isoelectric Point: 5.6556

>AT155620 WP_004085025.1 NZ_CP052854:c514711-514376 [Xylella fastidiosa subsp. multiplex]
MKNIMTIESFKAIITYDPEIDMFRGEFVGINGGADFYAKDLKGLRREGAISLKVFLEACKEDSVEPRKCYSGKFNARISP
ELHALASEAAAAQGISLNQFVEQAIQHEVHT

Download         Length: 336 bp

>AT155620 NZ_CP068809:1929689-1929940 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q87BV5

Antitoxin

Source ID Structure
AlphaFold DB A0A060HAK5

References