Detailed information of TA system TA155617

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 2367076..2367687
• Replicon: chromosome
• Accession: NZ_CP052853
• Organism: Xylella fastidiosa subsp. multiplex strain RH1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A060HHU8
• Locus tag: HKJ33_RS10845
• Protein ID: WP_024749166.1
• Coordinates: 2367076..2367375 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q879U0
• Locus tag: HKJ33_RS10850
• Protein ID: WP_004085003.1
• Coordinates: 2367379..2367687 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HKJ33_RS10810	2362292..2362852	-	561	WP_169710076.1	DUF2612 domain-containing protein	-
HKJ33_RS10815	2362849..2363994	-	1146	WP_004084997.1	baseplate J/gp47 family protein	-
HKJ33_RS10820	2364087..2364440	-	354	WP_004084998.1	hypothetical protein	-
HKJ33_RS10825	2364437..2365077	-	641	Protein_2118	hypothetical protein	-
HKJ33_RS10830	2365074..2365904	-	831	WP_169709942.1	hypothetical protein	-
HKJ33_RS10835	2365901..2366218	-	318	WP_169710077.1	hypothetical protein	-
HKJ33_RS10840	2366218..2366988	-	771	WP_169710078.1	hypothetical protein	-
HKJ33_RS10845	2367076..2367375	+	300	WP_024749166.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HKJ33_RS10850	2367379..2367687	+	309	WP_004085003.1	putative addiction module antidote protein	Antitoxin
HKJ33_RS10855	2367761..2369650	-	1890	WP_169710079.1	hypothetical protein	-
HKJ33_RS10860	2369798..2370220	-	423	WP_169710080.1	hypothetical protein	-
HKJ33_RS10865	2370217..2370654	-	438	WP_004085006.1	DUF3277 family protein	-
HKJ33_RS10870	2370664..2372160	-	1497	WP_004085007.1	DUF3383 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2357964..2403285	45321

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-98)

Antitoxin

• Uncharacterized protein HI1420 (15-100)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11364.14 Da
• Isoelectric Point: 10.3861

>T155617 WP_024749166.1 NZ_CP052853:2367076-2367375 [Xylella fastidiosa subsp. multiplex]
MTYTVKRLEGFSDWLKGLKDGLARQRLIKRLRKVQLGNFGDVQPVGEGVFEMREHFGPGWRMYYVQRGSFLIVMLGGGDK
STQQSDIRRAIELAKSLED

Nucleotide

• Length: 300 bp

>T155617 NZ_CP068809:974424-974831 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATTCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTACAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11060.61 Da
• Isoelectric Point: 4.6535

>AT155617 WP_004085003.1 NZ_CP052853:2367379-2367687 [Xylella fastidiosa subsp. multiplex]
VTITKKINVSELPEFDAAEYLNSEEEVAAYLTAVLEENDPALLAAALGDIARSRGMSQIAKDSGITREALYKALRPGSEP
RFDTISRVCTALGIRLVAQPMH

Nucleotide

• Length: 309 bp

>AT155617 NZ_CP068809:974177-974443 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A060HHU8

Antitoxin

• AlphaFold DB: A0A060HCD4