Detailed information of TA system TA155616

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 1781480..1782002
• Replicon: chromosome
• Accession: NZ_CP052853
• Organism: Xylella fastidiosa subsp. multiplex strain RH1

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: HKJ33_RS08255
• Protein ID: WP_004085905.1
• Coordinates: 1781715..1782002 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A060HDF9
• Locus tag: HKJ33_RS08250
• Protein ID: WP_004085758.1
• Coordinates: 1781480..1781722 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HKJ33_RS08220	1776890..1777072	+	183	WP_027700467.1	hypothetical protein	-
HKJ33_RS08225	1777356..1778282	-	927	WP_004085766.1	Grx4 family monothiol glutaredoxin	-
HKJ33_RS08230	1778348..1779124	+	777	WP_027700469.1	polysaccharide deacetylase family protein	-
HKJ33_RS08235	1779768..1779959	-	192	WP_021358405.1	hypothetical protein	-
HKJ33_RS08240	1780377..1780796	-	420	WP_004085763.1	putative toxin-antitoxin system toxin component, PIN family	-
HKJ33_RS08245	1780796..1781035	-	240	WP_004085761.1	ribbon-helix-helix domain-containing protein	-
HKJ33_RS08250	1781480..1781722	+	243	WP_004085758.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
HKJ33_RS08255	1781715..1782002	+	288	WP_004085905.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HKJ33_RS08260	1782090..1782656	+	567	WP_004085755.1	recombinase family protein	-
HKJ33_RS08265	1782709..1783905	-	1197	WP_004083925.1	transposase	-
HKJ33_RS08270	1783899..1784525	-	627	WP_038230952.1	IS607 family transposase	-
HKJ33_RS08275	1784755..1786029	-	1275	WP_012337985.1	pyridoxal phosphate-dependent aminotransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1782090..1782656	566
	-	inside	Genomic island	-	-	1780796..1801271	20475

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-90)

Antitoxin

• Antitoxin ParD (1-78)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11159.64 Da
• Isoelectric Point: 5.9570

>T155616 WP_004085905.1 NZ_CP052853:1781715-1782002 [Xylella fastidiosa subsp. multiplex]
MAKSYRLTPLAEADLEEIWFYTFRHWSIEQADSYHRSLVAVFEGLAAGTKQGCPSVLPDFNKYLCGSHVVYFMDYADHLD
IIRILHQRQDTERYL

Nucleotide

• Length: 288 bp

>T155616 NZ_CP068809:c830857-830483 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8774.77 Da
• Isoelectric Point: 6.9881

>AT155616 WP_004085758.1 NZ_CP052853:1781480-1781722 [Xylella fastidiosa subsp. multiplex]
MSRNTSVALGPHFTSFIDAQVQGGRYGTASDVVRAGLRLLEEHETKVKALQDALNVGHQSGEPRPFDSEVFLSRMHTQHG

Nucleotide

• Length: 243 bp

>AT155616 NZ_CP068809:c831315-830947 [Escherichia coli]
GTGTCAGACGCACTCTCCGGGACAATGCTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACAGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCTGACACTCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A060HDF9