Detailed information of TA system TA155615

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacT-ataR/DUF1778(antitoxin)
• Location: 1158507..1159261
• Replicon: chromosome
• Accession: NZ_CP052853
• Organism: Xylella fastidiosa subsp. multiplex strain RH1

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: HKJ33_RS05285
• Protein ID: WP_021358673.1
• Coordinates: 1158507..1158986 (-)
• Length: 160 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: -
• Locus tag: HKJ33_RS05290
• Protein ID: WP_004085396.1
• Coordinates: 1158989..1159261 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HKJ33_RS05275	1155361..1156167	+	807	WP_038211175.1	oxidoreductase	-
HKJ33_RS05280	1157201..1158171	+	971	Protein_1025	toprim domain-containing protein	-
HKJ33_RS05285	1158507..1158986	-	480	WP_021358673.1	GNAT family N-acetyltransferase	Toxin
HKJ33_RS05290	1158989..1159261	-	273	WP_004085396.1	DUF1778 domain-containing protein	Antitoxin
HKJ33_RS05295	1159613..1160518	-	906	WP_004085395.1	AEC family transporter	-
HKJ33_RS05300	1160634..1161252	+	619	Protein_1029	IS607 family transposase	-
HKJ33_RS05305	1161447..1163513	+	2067	WP_004085393.1	glycosyl hydrolase family 3	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (33-146)

Antitoxin

• Vibrio phage ICP1, Orf50 (3-85)

Sequences

Toxin

Protein

• Length: 160 a.a.
• Molecular weight: 17645.42 Da
• Isoelectric Point: 9.9283

>T155615 WP_021358673.1 NZ_CP052853:c1158986-1158507 [Xylella fastidiosa subsp. multiplex]
MQISTPHSLTAAHRLDEFNCGEPSLDDWLKRRALTNHLNGASRTFVVIDANQYVLGYYALAAGAVAHQEATRAIRRNMPD
PVPVMVLGRLAVDTRAQGIKLGAALLKDTVTRVQSIAENAGVRALVVHALNERAKQFYEYYGFRASPMHLMTLMLPIKF

Nucleotide

• Length: 480 bp

>T155615 NZ_CP068809:c609049-608585 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAGCCATTGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10264.77 Da
• Isoelectric Point: 5.0488

>AT155615 WP_004085396.1 NZ_CP052853:c1159261-1158989 [Xylella fastidiosa subsp. multiplex]
MRDASINLRAMPEQRDLIDQAANLLGKNRSDFMLEAACERAKAIILDQVFFNLDEERFRQFTVLLNAPQDTNPGLNRLMA
IKAPWSISNT

Nucleotide

• Length: 273 bp

>AT155615 NZ_CP068809:c609384-609049 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTAACGCTGTACTGACCACTGAATCAAAGGTCACTATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCGACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGACGATGAGAT
TGGCGACGACGAATAA