Detailed information of TA system TA155613

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 677570..678162
• Replicon: chromosome
• Accession: NZ_CP052853
• Organism: Xylella fastidiosa subsp. multiplex strain RH1

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A060HCK8
• Locus tag: HKJ33_RS02955
• Protein ID: WP_004085590.1
• Coordinates: 677570..677866 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: HKJ33_RS02960
• Protein ID: WP_004085592.1
• Coordinates: 677869..678162 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HKJ33_RS02925	672633..672932	+	300	WP_004085585.1	type II toxin-antitoxin system RelE/ParE family toxin	-
HKJ33_RS02930	672941..674055	+	1115	Protein_560	HigA family addiction module antidote protein	-
HKJ33_RS02935	674089..674568	+	480	WP_004085587.1	hypothetical protein	-
HKJ33_RS02940	674598..675761	-	1164	WP_169709939.1	phage tail protein	-
HKJ33_RS02945	675765..676325	-	561	WP_004084996.1	DUF2612 domain-containing protein	-
HKJ33_RS02950	676322..677467	-	1146	WP_169709940.1	baseplate J/gp47 family protein	-
HKJ33_RS02955	677570..677866	+	297	WP_004085590.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HKJ33_RS02960	677869..678162	+	294	WP_004085592.1	putative addiction module antidote protein	Antitoxin
HKJ33_RS02965	678218..678571	-	354	WP_169709941.1	hypothetical protein	-
HKJ33_RS02970	678568..679209	-	642	WP_004084999.1	hypothetical protein	-
HKJ33_RS02975	679206..680036	-	831	WP_169709942.1	hypothetical protein	-
HKJ33_RS02980	680033..680350	-	318	WP_012382585.1	hypothetical protein	-
HKJ33_RS02985	680350..681120	-	771	WP_169709943.1	hypothetical protein	-
HKJ33_RS02990	681208..681507	+	300	WP_024749166.1	type II toxin-antitoxin system RelE/ParE family toxin	-
HKJ33_RS02995	681511..681819	+	309	WP_004085003.1	putative addiction module antidote protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	674598..718839	44241

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-94)

Antitoxin

• Uncharacterized protein HI1420 (3-93)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11169.70 Da
• Isoelectric Point: 10.0610

>T155613 WP_004085590.1 NZ_CP052853:677570-677866 [Xylella fastidiosa subsp. multiplex]
MIELKQTDTFRKWREKLKDARARSAIASRLDRLAFGHVGDAEPVGKGVSELRINYGPGYRVYFQRRGDTIYLLLCGGDKG
SQARDIKTALHLSEQWSE

Nucleotide

• Length: 297 bp

>T155613 NZ_CP068808:c5092117-5091806 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10246.59 Da
• Isoelectric Point: 5.6232

>AT155613 WP_004085592.1 NZ_CP052853:677869-678162 [Xylella fastidiosa subsp. multiplex]
MTEKLTSYDPAEDLTTDQAIADFMAAAFGTNDPAYVAHALGVVARAKGMTQIASQTGLSREQLYRSFSAEGNPTLRTTLA
VMKALGIELSAKPSGVH

Nucleotide

• Length: 294 bp

>AT155613 NZ_CP068808:c5091805-5091515 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACACCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGAAC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACTGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAGGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A060HCK8