Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 513993..514579 Replicon chromosome
Accession NZ_CP052853
Organism Xylella fastidiosa subsp. multiplex strain RH1

Toxin (Protein)


Gene name hicA Uniprot ID Q87BV5
Locus tag HKJ33_RS02210 Protein ID WP_004089517.1
Coordinates 514325..514579 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID B2I6C9
Locus tag HKJ33_RS02205 Protein ID WP_004085025.1
Coordinates 513993..514328 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HKJ33_RS02175 510767..511342 - 576 WP_012337655.1 terminase small subunit -
HKJ33_RS02180 511473..511697 - 225 WP_012337656.1 hypothetical protein -
HKJ33_RS02185 511699..512160 - 462 WP_169709924.1 hypothetical protein -
HKJ33_RS02190 512150..512479 - 330 WP_004085022.1 phage holin family protein -
HKJ33_RS02195 512472..512972 - 501 WP_128382956.1 lysozyme -
HKJ33_RS02200 513072..513803 - 732 WP_169709925.1 site-specific DNA-methyltransferase -
HKJ33_RS02205 513993..514328 - 336 WP_004085025.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
HKJ33_RS02210 514325..514579 - 255 WP_004089517.1 type II toxin-antitoxin system HicA family toxin Toxin
HKJ33_RS02215 514632..515354 - 723 WP_012337661.1 hypothetical protein -
HKJ33_RS12245 516501..517100 + 600 WP_162008660.1 hypothetical protein -
HKJ33_RS02225 517097..517375 + 279 WP_038230497.1 VRR-NUC domain-containing protein -
HKJ33_RS02230 517377..517781 - 405 WP_004085029.1 hypothetical protein -
HKJ33_RS02235 517866..519284 + 1419 WP_169709926.1 DEAD/DEAH box helicase -
HKJ33_RS02240 519281..519445 + 165 WP_004086189.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 474721..523204 48483


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(23-80)

Antitoxin

Uncharacterised protein family HicB

(55-105)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 9639.14 Da        Isoelectric Point: 11.2766

>T155612 WP_004089517.1 NZ_CP052853:c514579-514325 [Xylella fastidiosa subsp. multiplex]
MKRKHAKTLSAIYTRPVSANIQWRDIEALFVELGAKVEEREGSRMLVRLFGERRIFHRPHPSPNTDKGAVESIRAWLKSN
GVTP

Download         Length: 255 bp

>T155612 NZ_CP068808:c4795998-4795825 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12324.07 Da        Isoelectric Point: 5.6556

>AT155612 WP_004085025.1 NZ_CP052853:c514328-513993 [Xylella fastidiosa subsp. multiplex]
MKNIMTIESFKAIITYDPEIDMFRGEFVGINGGADFYAKDLKGLRREGAISLKVFLEACKEDSVEPRKCYSGKFNARISP
ELHALASEAAAAQGISLNQFVEQAIQHEVHT

Download         Length: 336 bp

>AT155612 NZ_CP068808:c4796322-4796026 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCATTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q87BV5

Antitoxin

Source ID Structure
AlphaFold DB A0A060HAK5

References