Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-VagC
Location 2987448..2988121 Replicon chromosome
Accession NZ_CP052770
Organism Mesorhizobium japonicum strain R7ANSstar

Toxin (Protein)


Gene name vapC Uniprot ID -
Locus tag HKB47_RS14685 Protein ID WP_032930876.1
Coordinates 2987723..2988121 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A6I4AZ99
Locus tag HKB47_RS14680 Protein ID WP_032930877.1
Coordinates 2987448..2987723 (+) Length 92 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HKB47_RS14655 2982828..2983808 + 981 WP_032930881.1 ABC transporter permease -
HKB47_RS14660 2983805..2984791 + 987 WP_032930880.1 ABC transporter permease -
HKB47_RS14665 2984907..2985455 + 549 WP_032930879.1 gluconokinase -
HKB47_RS14670 2985539..2985988 - 450 WP_010910348.1 Rrf2 family transcriptional regulator -
HKB47_RS14675 2986237..2987223 - 987 WP_032930878.1 sulfate ABC transporter substrate-binding protein -
HKB47_RS14680 2987448..2987723 + 276 WP_032930877.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
HKB47_RS14685 2987723..2988121 + 399 WP_032930876.1 type II toxin-antitoxin system VapC family toxin Toxin
HKB47_RS14690 2988137..2989177 - 1041 WP_010910344.1 sulfate/molybdate ABC transporter ATP-binding protein -
HKB47_RS14695 2989190..2990074 - 885 WP_032930875.1 sulfate ABC transporter permease subunit CysW -
HKB47_RS14700 2990067..2990918 - 852 WP_010910342.1 sulfate ABC transporter permease subunit CysT -
HKB47_RS14705 2991000..2992028 - 1029 WP_032930874.1 sulfate ABC transporter substrate-binding protein -
HKB47_RS14710 2992233..2992964 - 732 WP_080680275.1 M15 family metallopeptidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(4-121)

Antitoxin

SpoVT-AbrB domain

(18-56)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14248.63 Da        Isoelectric Point: 5.7489

>T155588 WP_032930876.1 NZ_CP052770:2987723-2988121 [Mesorhizobium japonicum]
MRFMLDTNIISDMIRNPAGKAAGAMVREGDAAVCTSIVVASELRYGCARKGSTKLLKKVEDLLAEIPVLPLDVPVDAEYG
ALRAELEAVGQPIGHNDLFIAAHACVLGTTLVTANIGEFTRIRKLKVENWLE

Download         Length: 399 bp

>T155588 NZ_CP068808:259411-259518 [Escherichia coli]
ATGACGCTCGCAGAACTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CATGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 92 a.a.        Molecular weight: 10194.65 Da        Isoelectric Point: 4.8605

>AT155588 WP_032930877.1 NZ_CP052770:2987448-2987723 [Mesorhizobium japonicum]
MPQHRQTTTPPPKEAKLFRNNRSQAVRIPVEFELPGDKVLISREGDRLVIEPLRKPGLAALLAQWAKEAPLGPEDSFPEI
NDAPVEAEDIF

Download         Length: 276 bp

>AT155588 NZ_CP068808:c259362-259299 [Escherichia coli]
GTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A6I4AZ99

References