Detailed information of TA system TA155585

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-VagC
• Location: 2987441..2988114
• Replicon: chromosome
• Accession: NZ_CP052769
• Organism: Mesorhizobium japonicum strain R7AstarV2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HKB46_RS14680
• Protein ID: WP_032930876.1
• Coordinates: 2987716..2988114 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A6I4AZ99
• Locus tag: HKB46_RS14675
• Protein ID: WP_032930877.1
• Coordinates: 2987441..2987716 (+)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HKB46_RS14650	2982821..2983801	+	981	WP_032930881.1	ABC transporter permease	-
HKB46_RS14655	2983798..2984784	+	987	WP_032930880.1	ABC transporter permease	-
HKB46_RS14660	2984900..2985448	+	549	WP_032930879.1	gluconokinase	-
HKB46_RS14665	2985532..2985981	-	450	WP_010910348.1	Rrf2 family transcriptional regulator	-
HKB46_RS14670	2986230..2987216	-	987	WP_032930878.1	sulfate ABC transporter substrate-binding protein	-
HKB46_RS14675	2987441..2987716	+	276	WP_032930877.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HKB46_RS14680	2987716..2988114	+	399	WP_032930876.1	type II toxin-antitoxin system VapC family toxin	Toxin
HKB46_RS14685	2988130..2989170	-	1041	WP_010910344.1	sulfate/molybdate ABC transporter ATP-binding protein	-
HKB46_RS14690	2989183..2990067	-	885	WP_032930875.1	sulfate ABC transporter permease subunit CysW	-
HKB46_RS14695	2990060..2990911	-	852	WP_010910342.1	sulfate ABC transporter permease subunit CysT	-
HKB46_RS14700	2990993..2992021	-	1029	WP_032930874.1	sulfate ABC transporter substrate-binding protein	-
HKB46_RS14705	2992226..2992957	-	732	WP_080680275.1	M15 family metallopeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-121)

Antitoxin

• SpoVT-AbrB domain (18-56)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14248.63 Da
• Isoelectric Point: 5.7489

>T155585 WP_032930876.1 NZ_CP052769:2987716-2988114 [Mesorhizobium japonicum]
MRFMLDTNIISDMIRNPAGKAAGAMVREGDAAVCTSIVVASELRYGCARKGSTKLLKKVEDLLAEIPVLPLDVPVDAEYG
ALRAELEAVGQPIGHNDLFIAAHACVLGTTLVTANIGEFTRIRKLKVENWLE

Nucleotide

• Length: 399 bp

>T155585 NZ_CP068808:202759-202944 [Escherichia coli]
GTGAAAAGTGCAGACGTGATCGCCATCCTGAAGCAGCATGGCTGGGAACATATTCGAACCCGTGGCAGTCATCATCAGTT
CCGCCACCCTGTTCATCCGGGTCTGGTGACGGTTCCGCACCCTAAAAAAGATATTAAGCCCGGTACGCTGGCGCAAATTG
GGCGTCAGGCTGGTTTAACATTTTAA

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10194.65 Da
• Isoelectric Point: 4.8605

>AT155585 WP_032930877.1 NZ_CP052769:2987441-2987716 [Mesorhizobium japonicum]
MPQHRQTTTPPPKEAKLFRNNRSQAVRIPVEFELPGDKVLISREGDRLVIEPLRKPGLAALLAQWAKEAPLGPEDSFPEI
NDAPVEAEDIF

Nucleotide

• Length: 276 bp

>AT155585 NZ_CP068808:202961-203371 [Escherichia coli]
ATGTTTTATCCCGCCTATATCCACAGTGATCCTGACGGCAGCGCCAGCGGTTTTTTCCCTGATGTTCCAGGCTGTTATTT
TGCTGGTGATACGCTGGACAACGCTTTCCAGGATGCGCGAAGTGCACTGGTCGCGCACTTCGAAACCTTATGCGAGATGA
ATGAAGAGTTGCCGCTTCCAGGCTCTGTGGAAACACATCTGGTCCAGCGTGCGCAGGACTTTATTGGTGGGCAATGGTTG
CTGGTGGATATCAATATGAATCAGTTCGAAGGTCGGGCTGAACGTATCAATATTACGATGCCGAAAAGGTTACTGAACAA
GATTGATACCTACGTGCGCAATAACCCAGACTACGCCAACAGAAGTGCGTTTCTGGCAGAAGCTGCCCGGCGTGTATTGC
CGGGCGTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A6I4AZ99