Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicB-HicA
Location 2643824..2644419 Replicon chromosome
Accession NZ_CP052769
Organism Mesorhizobium japonicum strain R7AstarV2

Toxin (Protein)


Gene name hicA Uniprot ID A0A4Q1UJ83
Locus tag HKB46_RS12910 Protein ID WP_065141637.1
Coordinates 2644231..2644419 (-) Length 63 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID Q98J17
Locus tag HKB46_RS12905 Protein ID WP_010910701.1
Coordinates 2643824..2644225 (-) Length 134 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HKB46_RS12880 2639119..2640414 - 1296 WP_032931139.1 TIGR01244 family phosphatase -
HKB46_RS12885 2640790..2641242 + 453 WP_032931138.1 SRPBCC domain-containing protein -
HKB46_RS12890 2641271..2642101 - 831 WP_032931136.1 helix-turn-helix transcriptional regulator -
HKB46_RS12895 2642192..2643076 + 885 WP_032931134.1 SDR family oxidoreductase -
HKB46_RS12900 2643089..2643787 - 699 WP_032931133.1 RibD family protein -
HKB46_RS12905 2643824..2644225 - 402 WP_010910701.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
HKB46_RS12910 2644231..2644419 - 189 WP_065141637.1 type II toxin-antitoxin system HicA family toxin Toxin
HKB46_RS12915 2644780..2645727 + 948 WP_032931132.1 substrate-binding domain-containing protein -
HKB46_RS12920 2645970..2647568 + 1599 WP_032931131.1 sugar ABC transporter ATP-binding protein -
HKB46_RS12925 2647565..2648581 + 1017 WP_010910697.1 ABC transporter permease -
HKB46_RS12930 2648616..2649122 - 507 WP_080680478.1 histidine phosphatase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(4-59)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(4-124)


Sequences

Toxin        


Download         Length: 63 a.a.        Molecular weight: 7148.23 Da        Isoelectric Point: 11.6118

>T155584 WP_065141637.1 NZ_CP052769:c2644419-2644231 [Mesorhizobium japonicum]
MNSTEIIRQLQADGWFEVARKGSHAQFKHRDKQGRVTVPHPKRDIPIGTLRSIEKQSGLKLR

Download         Length: 189 bp

>T155584 NZ_CP068808:c72958-72581 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTCACGCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCATTTGTCCCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCGA
CAATTACAGAATGGTAAATAACATTACCCAGGGTAAGCATCCGGAGGCGCAACAATGA

Antitoxin


Download         Length: 134 a.a.        Molecular weight: 14185.00 Da        Isoelectric Point: 4.3919

>AT155584 WP_010910701.1 NZ_CP052769:c2644225-2643824 [Mesorhizobium japonicum]
MKQYIGLIHKDAGSDFGVSFPDFPGVITAGKDLDDARSMAEEALTLHIEGLVEDGEAIPEPSSLQAVMADADNRDGVAIL
VAAKTEARKAVRINVTLPDDVLRRIDAFAEAHGYTRSGFLAKAAEKAMELESA

Download         Length: 402 bp

>AT155584 NZ_CP068808:c73416-73048 [Escherichia coli]
GTGTCAGACACACTGCCCGGGACAACGCATCCCGACGACAATCACGACCGCCTCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCAATGCAGATGCATACCACCTGGACCAGGCCTTTGCACTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4Q1UJ83

Antitoxin

Source ID Structure
AlphaFold DB A0A4Q1UK54

References