Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5042115..5042740 Replicon chromosome
Accession NZ_CP052761
Organism Klebsiella pneumoniae strain XH210

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag RJ97_RS24555 Protein ID WP_002882817.1
Coordinates 5042115..5042498 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag RJ97_RS24560 Protein ID WP_004150355.1
Coordinates 5042498..5042740 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RJ97_RS24540 (5039481) 5039481..5040383 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
RJ97_RS24545 (5040380) 5040380..5041015 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
RJ97_RS24550 (5041012) 5041012..5041941 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
RJ97_RS24555 (5042115) 5042115..5042498 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
RJ97_RS24560 (5042498) 5042498..5042740 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
RJ97_RS24565 (5042945) 5042945..5043862 + 918 WP_023302328.1 alpha/beta hydrolase -
RJ97_RS24570 (5043876) 5043876..5044817 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
RJ97_RS24575 (5044862) 5044862..5045299 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
RJ97_RS24580 (5045296) 5045296..5046156 - 861 WP_004146232.1 virulence factor BrkB family protein -
RJ97_RS24585 (5046150) 5046150..5046749 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T155579 WP_002882817.1 NZ_CP052761:c5042498-5042115 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T155579 NZ_CP068807:c4780333-4779947 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT155579 WP_004150355.1 NZ_CP052761:c5042740-5042498 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT155579 NZ_CP068807:c4779957-4779628 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCATTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References