Detailed information of TA system TA155576

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 4495400..4496091
• Replicon: chromosome
• Accession: NZ_CP052761
• Organism: Klebsiella pneumoniae strain XH210

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: A0A483YFJ1
• Locus tag: RJ97_RS21995
• Protein ID: WP_025987721.1
• Coordinates: 4495400..4495741 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: RJ97_RS22000
• Protein ID: WP_038434619.1
• Coordinates: 4495765..4496091 (-)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RJ97_RS21980 (4492399)	4492399..4493634	-	1236	WP_068854594.1	hypothetical protein	-
RJ97_RS21985 (4494053)	4494053..4494385	-	333	WP_074439534.1	hypothetical protein	-
RJ97_RS21990 (4494452)	4494452..4495285	-	834	WP_038434618.1	DUF4942 domain-containing protein	-
RJ97_RS21995 (4495400)	4495400..4495741	-	342	WP_025987721.1	TA system toxin CbtA family protein	Toxin
RJ97_RS22000 (4495765)	4495765..4496091	-	327	WP_038434619.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
RJ97_RS22005 (4496106)	4496106..4496582	-	477	WP_038434581.1	DNA repair protein RadC	-
RJ97_RS22010 (4496593)	4496593..4497039	-	447	WP_038434582.1	antirestriction protein	-
RJ97_RS22015 (4497263)	4497263..4497952	-	690	WP_038434583.1	hypothetical protein	-
RJ97_RS22020 (4498272)	4498272..4498625	+	354	WP_038434584.1	hypothetical protein	-
RJ97_RS22025 (4498662)	4498662..4499552	-	891	WP_038434585.1	YfjP family GTPase	-
RJ97_RS22030 (4499624)	4499624..4500712	-	1089	WP_038434586.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4492399..4511663	19264

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-107)

Antitoxin

• Antitoxin CbeA (8-107)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12867.90 Da
• Isoelectric Point: 8.5195

>T155576 WP_025987721.1 NZ_CP052761:c4495741-4495400 [Klebsiella pneumoniae]
MKTLPATIQRAAKPCPSPVTVWQMLLTRLLEQHYGLTLNDTPFSDETVIQEHINAGITLVDAVNFLVEKYELVRIDRRGF
NCQEQSPYLGAVDILRARQATGLLRQRHLPSIR

Nucleotide

• Length: 342 bp

>T155576 NZ_CP068807:4157853-4158251 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12304.06 Da
• Isoelectric Point: 6.9560

>AT155576 WP_038434619.1 NZ_CP052761:c4496091-4495765 [Klebsiella pneumoniae]
MPLTDAIQWGLKRSFTPNFGARLVQEGNRLHYLADRANITGKFGDVECLKLEEAFPHFIRQMELMLSTGELNPRHAHSVT
LYHNAFTCEADTLGSCGYVYIVIYPTQR

Nucleotide

• Length: 327 bp

>AT155576 NZ_CP068807:4157557-4157850 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAATCCAGCAAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAACTGGTGAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A483YFJ1