Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4462648..4463458 Replicon chromosome
Accession NZ_CP052761
Organism Klebsiella pneumoniae strain XH210

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag RJ97_RS21845 Protein ID WP_004178461.1
Coordinates 4462648..4463181 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag RJ97_RS21850 Protein ID WP_002887278.1
Coordinates 4463192..4463458 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RJ97_RS21840 (4461479) 4461479..4462600 + 1122 WP_012737335.1 cupin domain-containing protein -
RJ97_RS21845 (4462648) 4462648..4463181 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
RJ97_RS21850 (4463192) 4463192..4463458 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
RJ97_RS21855 (4463561) 4463561..4464994 - 1434 WP_029498528.1 Cu(+)/Ag(+) sensor histidine kinase -
RJ97_RS21860 (4464984) 4464984..4465667 - 684 WP_023302433.1 copper response regulator transcription factor CusR -
RJ97_RS21865 (4465839) 4465839..4467224 + 1386 WP_023302432.1 efflux transporter outer membrane subunit -
RJ97_RS21870 (4467242) 4467242..4467586 + 345 WP_004214146.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T155575 WP_004178461.1 NZ_CP052761:c4463181-4462648 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T155575 NZ_CP068807:3813710-3813919 [Escherichia coli]
ATGGTTGATTATCCAAAACGTGATGCTATTTCCAACATCAACGCTATAATTGTAGATACATTTTTGTATATACAATTAGG
GATGGCAACTATGCCAATGGAGTTTGAATGGAATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTG
AAGATGCGGTGTTAGTGCTTGATGATCCACAACGAACGGAGCCGCTATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT155575 WP_002887278.1 NZ_CP052761:c4463458-4463192 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT155575 NZ_CP068807:3813916-3814218 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGTGCTGGCGAA
CAAATTCGACGAGGATATTGATTACAGCGACATCCCATCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References