Detailed information of TA system TA155558

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 5407343..5407929
• Replicon: chromosome
• Accession: NZ_CP052759
• Organism: Pseudomonas aeruginosa strain LYT4

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: HH306_RS25475
• Protein ID: WP_003120987.1
• Coordinates: 5407630..5407929 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: HH306_RS25470
• Protein ID: WP_003448662.1
• Coordinates: 5407343..5407633 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HH306_RS25450	5402494..5402703	+	210	WP_003105733.1	cold-shock protein	-
HH306_RS25455	5402925..5404814	+	1890	WP_016851610.1	hypothetical protein	-
HH306_RS25460	5404811..5406787	+	1977	WP_010792226.1	DEAD/DEAH box helicase	-
HH306_RS25465	5406928..5407272	+	345	WP_016851612.1	hypothetical protein	-
HH306_RS25470	5407343..5407633	-	291	WP_003448662.1	putative addiction module antidote protein	Antitoxin
HH306_RS25475	5407630..5407929	-	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HH306_RS25480	5408131..5409255	+	1125	WP_012076859.1	TcpQ domain-containing protein	-
HH306_RS25485	5409255..5410964	+	1710	WP_034037331.1	PilN family type IVB pilus formation outer membrane protein	-
HH306_RS25490	5410968..5412293	+	1326	WP_031806139.1	type 4b pilus protein PilO2	-
HH306_RS25495	5412283..5412816	+	534	WP_023094340.1	type IV pilus biogenesis protein PilP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	5391142..5407929	16787

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T155558 WP_003120987.1 NZ_CP052759:c5407929-5407630 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T155558 NZ_CP068807:c970000-969749 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.91 Da
• Isoelectric Point: 4.7527

>AT155558 WP_003448662.1 NZ_CP052759:c5407633-5407343 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT155558 NZ_CP068807:c969752-969498 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• AlphaFold DB: G8CP73