Detailed information of TA system TA155552

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazF-pemI/MazF(toxin)
• Location: 537614..538247
• Replicon: chromosome
• Accession: NZ_CP052135
• Organism: Bacillus sp. CS13

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: HHK01_RS02595
• Protein ID: WP_170006302.1
• Coordinates: 537897..538247 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: HHK01_RS02590
• Protein ID: WP_170006301.1
• Coordinates: 537614..537892 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHK01_RS02570	533574..534170	-	597	WP_170006297.1	rhomboid family intramembrane serine protease	-
HHK01_RS02575	534481..534843	+	363	WP_170006298.1	holo-ACP synthase	-
HHK01_RS02580	535122..536138	+	1017	WP_170006299.1	outer membrane lipoprotein carrier protein LolA	-
HHK01_RS02585	536247..537386	+	1140	WP_170006300.1	alanine racemase	-
HHK01_RS02590	537614..537892	+	279	WP_170006301.1	antitoxin endoai	Antitoxin
HHK01_RS02595	537897..538247	+	351	WP_170006302.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
HHK01_RS02600	538574..538975	+	402	WP_170006303.1	ATP-binding protein	-
HHK01_RS02605	538988..540004	+	1017	WP_170006304.1	PP2C family protein-serine/threonine phosphatase	-
HHK01_RS02610	540063..540395	+	333	WP_170006305.1	anti-sigma factor antagonist	-
HHK01_RS02615	540392..540865	+	474	WP_170008627.1	anti-sigma B factor RsbW	-
HHK01_RS02620	540843..541619	+	777	WP_170006306.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-113)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13034.04 Da
• Isoelectric Point: 5.1771

>T155552 WP_170006302.1 NZ_CP052135:537897-538247 [Bacillus sp. CS13]
MNVKRGDVYFADLSPVVGSEQGGVRPVLIIQNDIGNRFSPTVIIAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITQLDEEMMEKVDEAVQISLGLVHF

Nucleotide

• Length: 351 bp

>T155552 NZ_CP068807:c122057-121863 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10833.35 Da
• Isoelectric Point: 7.3178

>AT155552 WP_170006301.1 NZ_CP052135:537614-537892 [Bacillus sp. CS13]
VSESKATTEISIRLPEKMVAELDHFVQDHKDRDELIYRATKMYLRERKKRQIRESMRRGYMEMAKINLSLASEAFQAEYE
AEHTVERLVSGG

Nucleotide

• Length: 279 bp

>AT155552 NZ_CP068807:121686-121864 [Escherichia coli]
GAGGACTGAAGTCTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGGCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT