Detailed information of TA system TA155550

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapC-vagC/VapC-VagC
• Location: 216685..217322
• Replicon: plasmid pbghn2
• Accession: NZ_CP052134
• Organism: Burkholderia glumae strain HN

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: C5AP38
• Locus tag: GAS18_RS28250
• Protein ID: WP_012735347.1
• Coordinates: 216921..217322 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: GAS18_RS28245
• Protein ID: WP_017924531.1
• Coordinates: 216685..216921 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GAS18_RS28230	211985..213820	+	1836	WP_153479182.1	site-specific integrase	-
GAS18_RS28235	213905..214330	+	426	WP_171467078.1	hypothetical protein	-
GAS18_RS28240	214544..216253	+	1710	WP_153479184.1	MCP four helix bundle domain-containing protein	-
GAS18_RS28245	216685..216921	+	237	WP_017924531.1	AbrB family transcriptional regulator	Antitoxin
GAS18_RS28250	216921..217322	+	402	WP_012735347.1	type II toxin-antitoxin system VapC family toxin	Toxin
GAS18_RS28255	217571..217900	+	330	WP_155297075.1	hypothetical protein	-
GAS18_RS28260	217918..218163	+	246	WP_171467079.1	hypothetical protein	-
GAS18_RS28265	218204..218932	-	729	WP_012735342.1	IS6 family transposase	-
GAS18_RS28270	218975..219619	+	645	Protein_204	site-specific integrase	-
GAS18_RS28275	219704..220129	+	426	WP_012734109.1	hypothetical protein	-
GAS18_RS28280	220392..220643	+	252	WP_012734108.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
GAS18_RS28285	220637..220987	+	351	WP_012734107.1	type II toxin-antitoxin system ChpB family toxin	-
GAS18_RS28290	221046..221240	+	195	WP_124837685.1	hypothetical protein	-
GAS18_RS28295	221390..221611	+	222	WP_017433768.1	hypothetical protein	-
GAS18_RS28300	221834..222298	+	465	WP_012734106.1	Lrp/AsnC family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	tsr / tsr	1..281656	281656

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-117)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14847.12 Da
• Isoelectric Point: 5.2138

>T155550 WP_012735347.1 NZ_CP052134:216921-217322 [Burkholderia glumae]
MPRFMLDTNMCIYLMKNQPEQVAKRFAECFVGDVVMSAITYAELEYGVAVSDNRAKERRNLAALIEDILVAPFDAAAAVA
YGPIREATRERKKDHLDKLIAAHAVALDVVLVTNNAKDFASYPGIKLDNWLND

Nucleotide

• Length: 402 bp

>T155550 NZ_CP068806:4223671-4224135 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCTTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGGCAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8965.10 Da
• Isoelectric Point: 6.3676

>AT155550 WP_017924531.1 NZ_CP052134:216685-216921 [Burkholderia glumae]
MQTTRVFRNGNSQAVRIPADLAYERSDVELEIERVGDEIRIRPARRPLTGVLKKFAKFSQDFMAEGRGEQEQAEREGL

Nucleotide

• Length: 237 bp

>AT155550 NZ_CP068806:4223336-4223671 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACACC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: C5AP38