Detailed information of TA system TA155549

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): slvAT/RES(toxin)
• Location: 209187..210142
• Replicon: plasmid pbghn2
• Accession: NZ_CP052134
• Organism: Burkholderia glumae strain HN

Toxin (Protein)

• Gene name: slvT
• Uniprot ID: -
• Locus tag: GAS18_RS28200
• Protein ID: WP_017924249.1
• Coordinates: 209609..210142 (+)
• Length: 178 a.a.

Antitoxin (Protein)

• Gene name: slvA
• Uniprot ID: -
• Locus tag: GAS18_RS28195
• Protein ID: WP_017924250.1
• Coordinates: 209187..209612 (+)
• Length: 142 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GAS18_RS28175	204286..205143	-	858	WP_012734037.1	replication initiator protein A	-
GAS18_RS28180	205898..206863	-	966	WP_012734036.1	ParB/RepB/Spo0J family partition protein	-
GAS18_RS28185	206863..207534	-	672	WP_012734035.1	AAA family ATPase	-
GAS18_RS28190	208855..209040	+	186	WP_171467077.1	hypothetical protein	-
GAS18_RS28195	209187..209612	+	426	WP_017924250.1	DUF2384 domain-containing protein	Antitoxin
GAS18_RS28200	209609..210142	+	534	WP_017924249.1	RES family NAD+ phosphorylase	Toxin
GAS18_RS28205	210303..210500	+	198	WP_127840952.1	hypothetical protein	-
GAS18_RS28210	210762..211046	+	285	WP_017924445.1	hypothetical protein	-
GAS18_RS28215	211059..211298	+	240	WP_017924444.1	hypothetical protein	-
GAS18_RS28220	211295..211540	+	246	WP_153479180.1	hypothetical protein	-
GAS18_RS28225	211641..211982	+	342	WP_039204389.1	hypothetical protein	-
GAS18_RS28230	211985..213820	+	1836	WP_153479182.1	site-specific integrase	-
GAS18_RS28235	213905..214330	+	426	WP_171467078.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	tsr / tsr	1..281656	281656

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (11-168)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 178 a.a.
• Molecular weight: 19131.69 Da
• Isoelectric Point: 5.1753

>T155549 WP_017924249.1 NZ_CP052134:209609-210142 [Burkholderia glumae]
MILTPLQDITAYRMHVPKWAVAPTSGAGAARAGGRANRVGTPALYLALEVATAVAEYQQLSPLLPPGTLVSYRVTATPVV
DFTGGFRADLWPPLWESFFCDWRRDWFNARIEPPSWVLGDEAIAAGAKGILFRSTQATGGTNLVLFVDQLGEGDRLDVHD
PAGALPRDQASWDGPSR

Nucleotide

• Length: 534 bp

>T155549 NZ_CP068806:c4181087-4180773 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAATCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTCGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCCGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 142 a.a.
• Molecular weight: 14935.78 Da
• Isoelectric Point: 6.4664

>AT155549 WP_017924250.1 NZ_CP052134:209187-209612 [Burkholderia glumae]
MSTLAATRAPAAPAGGPAPASFDAFLSALREPDTAAPVLSARRYVAALNIDLQTLADQAHVHRNTVARAPQSASVQQFLR
EAIRVIRAATDLSGDVQRALFWYRNEPLAVFDYQTAETLVSAGRADDVVRYVASLEAGAAG

Nucleotide

• Length: 426 bp

>AT155549 NZ_CP068806:c4180776-4180360 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACGCCCAGGCGCTGGAACTGGTCGATCATCTGCTGCTCAACGATCCTGAAAATCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCTGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTTCCGGAAATTGGCAGTAAATCCATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAACTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA