Detailed information of TA system TA155545

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-MazE
• Location: 527836..528469
• Replicon: chromosome
• Accession: NZ_CP052130
• Organism: Ralstonia solanacearum strain FJAT1303.F8

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HI794_RS02455
• Protein ID: WP_173941463.1
• Coordinates: 528056..528469 (+)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q8XUX7
• Locus tag: HI794_RS02450
• Protein ID: WP_011002949.1
• Coordinates: 527836..528069 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HI794_RS02435	523424..524719	-	1296	WP_011002952.1	MFS transporter	-
HI794_RS02440	524727..525977	-	1251	WP_089190599.1	AGE family epimerase/isomerase	-
HI794_RS02445	526172..527779	+	1608	WP_058908048.1	glucan biosynthesis protein	-
HI794_RS02450	527836..528069	+	234	WP_011002949.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HI794_RS02455	528056..528469	+	414	WP_173941463.1	PIN domain-containing protein	Toxin
HI794_RS02460	528444..529070	-	627	WP_173941464.1	DUF1415 family protein	-
HI794_RS02465	529261..529644	+	384	WP_173941465.1	hypothetical protein	-
HI794_RS02470	529706..531202	-	1497	WP_173941466.1	glycerol kinase GlpK	-
HI794_RS02475	531368..533104	-	1737	WP_173941467.1	ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-116)

Antitoxin

• SpoVT-AbrB domain (5-37)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 15046.37 Da
• Isoelectric Point: 5.5348

>T155545 WP_173941463.1 NZ_CP052130:528056-528469 [Ralstonia solanacearum]
MPATEAFFDSNVVLYLLSADTAKADAAETLLMTGGVVSVQVLNETTHVMRRKLAMPWHAIETVQEAVRAQCRVEPLTLET
HDLGRRIAERYGLSVYDALIVAAALLAGCNVLYSEDMQHGLVIEPHLRIVNPFSARA

Nucleotide

• Length: 414 bp

>T155545 NZ_CP068806:c2362022-2360700 [Escherichia coli]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAATGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCCAACCGTTATGCCAGACCGTTATCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCCAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATACCCGAAGA
CGAAACCGTAACGCATCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCTCTGGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCGCAGGAGAAGACAGCGCTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTGCCGGACGCAGAAATCATTAAAGCGGGAAATGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATGCTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAGTTTCAGGTGTTCCAGTGGTTGATTGGCGCAACGGATGGCCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTATTGGGTGGCGCAGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTCTGGCGACAGCAAAGGTGCTGAAATTTCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCG
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAACCAATGTGTT
GAGGTTGCACCGTCGCTTAAGCCGAGAATACGGTATTAAGTAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8790.98 Da
• Isoelectric Point: 8.7287

>AT155545 WP_011002949.1 NZ_CP052130:527836-528069 [Ralstonia solanacearum]
MQVSKWGNSLAIRLPAAVVEALQLHEGDDVEVVIAGERSFGVRRKRAARELFDRIHQYRGKLPADFIFERDQANARD

Nucleotide

• Length: 234 bp

>AT155545 NZ_CP068806:c2362288-2362022 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTAGCGAATGCAATGAAACTGGTTCGTCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATCGGTATTAAGCAGGCGACAATTTCCAATTTCGAAAACAATCCTGACAATACCACGC
TGACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACACGAAAAATGCCTCGCCAGAATCA
ACAGAACAGCAAGATCTGGAGTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A223GR76