Detailed information of TA system TA155543

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/COG3657-dnstrm_HI1420
• Location: 49584..50113
• Replicon: plasmid Plas1
• Accession: NZ_CP052129
• Organism: Ralstonia solanacearum strain FJAT1303.F1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: HI792_RS17305
• Protein ID: WP_011003396.1
• Coordinates: 49886..50113 (-)
• Length: 76 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A0S4U6E5
• Locus tag: HI792_RS17300
• Protein ID: WP_064478231.1
• Coordinates: 49584..49889 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HI792_RS17270	45086..45571	+	486	WP_016726511.1	hypothetical protein	-
HI792_RS17275	45902..46183	-	282	WP_016722336.1	hypothetical protein	-
HI792_RS17280	46314..46598	-	285	WP_011003391.1	hypothetical protein	-
HI792_RS17285	46931..47118	+	188	Protein_44	IS5/IS1182 family transposase	-
HI792_RS17290	47285..47953	+	669	WP_038938024.1	hypothetical protein	-
HI792_RS17295	48058..49371	-	1314	WP_064478230.1	metabolite/H+ symporter	-
HI792_RS17300	49584..49889	-	306	WP_064478231.1	putative addiction module antidote protein	Antitoxin
HI792_RS17305	49886..50113	-	228	WP_011003396.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HI792_RS17310	50269..51468	-	1200	WP_071623818.1	MFS transporter	-
HI792_RS17315	51595..52416	-	822	WP_011003398.1	GntR family transcriptional regulator	-
HI792_RS17320	52594..53145	-	552	WP_011003399.1	helix-turn-helix transcriptional regulator	-
HI792_RS17325	53278..53877	+	600	WP_064478235.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	cyaB / flgC / flgG / fliM / fliG / fliI / adeG / acrB / cheY / cheB / cheW / motA	1..2051206	2051206

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-62)

Antitoxin

• Cro/C1-type helix-turn-helix domain (52-96)

Sequences

Toxin

Protein

• Length: 76 a.a.
• Molecular weight: 8544.80 Da
• Isoelectric Point: 6.4584

>T155543 WP_011003396.1 NZ_CP052129:c50113-49886 [Ralstonia solanacearum]
VRIDRLQMGNPGDMKAVRDGIRELRIDHGPGDRLYFVRHGVVLIVLLCGGDKSTQETDIRRAIDLSRRLDVDDME

Nucleotide

• Length: 228 bp

>T155543 NZ_CP068806:2261928-2262104 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTGGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCAAGGGCGACGCAGTGTCATGCCGCGTCACCCCAGTGATGAGATAAAAGAACCATTGCGTAAAGCAATCCTGAAGC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 10983.62 Da
• Isoelectric Point: 7.2780

>AT155543 WP_064478231.1 NZ_CP052129:c49889-49584 [Ralstonia solanacearum]
MTMAQRTIKTRLWDSAEHLRTEADIAAYLDACLEEAGDDPAFITHALGVVARSRGMTQLARETGMTREGLYKALSEGGNP
SFATVLKVIRALGIRLHAVPT

Nucleotide

• Length: 306 bp

>AT155543 NZ_CP068806:2262150-2262566 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACAGTTGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ATGAGCTAATCCCGTTGCCTTCGCCATTAACTAACAACGATCACTTTATTGAAGTGCCTTTAAGTATCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGACGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAATTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A0S4U6E5