Detailed information of TA system TA155529

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 2257998..2259593
• Replicon: chromosome
• Accession: NZ_CP052120
• Organism: Ralstonia solanacearum strain FJAT1458.F1

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A0S4UDK5
• Locus tag: HI797_RS10645
• Protein ID: WP_069079583.1
• Coordinates: 2258256..2259593 (+)
• Length: 446 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A0S4UDK3
• Locus tag: HI797_RS10640
• Protein ID: WP_016725279.1
• Coordinates: 2257998..2258252 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HI797_RS10615	2254061..2254987	-	927	WP_016727232.1	muramoyltetrapeptide carboxypeptidase	-
HI797_RS10620	2254984..2255535	-	552	WP_016727233.1	tRNA adenosine(34) deaminase TadA	-
HI797_RS10625	2255555..2256031	-	477	WP_011001399.1	6-carboxytetrahydropterin synthase QueD	-
HI797_RS10630	2256099..2256737	-	639	WP_020831956.1	7-carboxy-7-deazaguanine synthase	-
HI797_RS10635	2257055..2257894	+	840	WP_016725278.1	oxaloacetate decarboxylase	-
HI797_RS10640	2257998..2258252	+	255	WP_016725279.1	helix-turn-helix transcriptional regulator	Antitoxin
HI797_RS10645	2258256..2259593	+	1338	WP_069079583.1	type II toxin-antitoxin system HipA family toxin	Toxin
HI797_RS10650	2259590..2260792	+	1203	WP_064047701.1	MFS transporter	-
HI797_RS10655	2261018..2261746	+	729	WP_011001393.1	DsbA family protein	-
HI797_RS10660	2261759..2262322	-	564	WP_080606483.1	RNA 2',3'-cyclic phosphodiesterase	-
HI797_RS10665	2262503..2263201	-	699	WP_016725453.1	protocatechuate 3,4-dioxygenase subunit alpha	-
HI797_RS10670	2263198..2263938	-	741	WP_011001390.1	protocatechuate 3,4-dioxygenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (6-107)

Antitoxin

• Transcription regulator, HipB-like (10-66)

Sequences

Toxin

Protein

• Length: 446 a.a.
• Molecular weight: 48724.75 Da
• Isoelectric Point: 6.7232

>T155529 WP_069079583.1 NZ_CP052120:2258256-2259593 [Ralstonia solanacearum]
MAHPELHLWMNGEHVGIWTASRAGVPILRYDDGWLRSPNMRPLSLSLPIPAGVPELRGQVVDDYFDNLLPEAQPIRERVR
RRFGLASTDAAELLAAIGRDCVGAVQLLPPGMAPTGYDRIDSEPLTDSQVDTLLRNLTATPALGEDAGDPDAFRISIAGA
QEKTALLRIGDAWHRPLGATPTTHILKLPLGLIGNIRADMQDSVDNEWLCAQLLAAWGLEVARTEIARFGASKVLAVERF
DRRWMPGNAWIARLPQEDFCQATGTPAHRKYENDGGPGMRACLDLLSASEHAIDDKRRFVLAQLAFWLLAATDGHAKNFS
LAIGRGGRFRMTPLYDVLSVWPIMGDGPNQLSPYRARLAMALRGKNAHARLADIHVRHWQALAAQSGVPDAFAGMQALVE
RVPAGLERVEAILPPDFSGRVWSSVRTGVLAQAERFLNELASVTP

Nucleotide

• Length: 1338 bp

>T155529 NZ_CP068805:c4595841-4595491 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGTGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGGTGAGGTGGTGGAAGAGGC
GTTATTACGTTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 8754.07 Da
• Isoelectric Point: 10.4693

>AT155529 WP_016725279.1 NZ_CP052120:2257998-2258252 [Ralstonia solanacearum]
MESLLSSAPQLSAHLKSLRKARGLTQAQLGALLGVSQNRIADIEKDPGAVGFEQLLRILHALGAQLTLRTSAPAGTPSAA
PADW

Nucleotide

• Length: 255 bp

>AT155529 NZ_CP068805:c4596086-4595835 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCACGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: A0A0S4UDK5

Antitoxin

• AlphaFold DB: A0A0S4UDK3