Detailed information of TA system TA155520

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/COG3657-dnstrm_HI1420
• Location: 65813..66342
• Replicon: plasmid Plas1
• Accession: NZ_CP052117
• Organism: Ralstonia solanacearum strain FJAT1463.F1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: HI799_RS19265
• Protein ID: WP_016722340.1
• Coordinates: 66115..66342 (-)
• Length: 76 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: HI799_RS19260
• Protein ID: WP_016722339.1
• Coordinates: 65813..66118 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HI799_RS19230	60917..61198	-	282	WP_016722336.1	hypothetical protein	-
HI799_RS19235	61329..61613	-	285	WP_011003391.1	hypothetical protein	-
HI799_RS19240	62065..63051	+	987	WP_016721870.1	IS5 family transposase	-
HI799_RS19245	63196..63347	+	152	Protein_58	IS5/IS1182 family transposase	-
HI799_RS19250	63514..64182	+	669	WP_038938024.1	hypothetical protein	-
HI799_RS19255	64287..65600	-	1314	WP_038938025.1	metabolite/H+ symporter	-
HI799_RS19260	65813..66118	-	306	WP_016722339.1	putative addiction module antidote protein	Antitoxin
HI799_RS19265	66115..66342	-	228	WP_016722340.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HI799_RS19270	66497..67696	-	1200	WP_016726513.1	MFS transporter	-
HI799_RS19275	67823..68644	-	822	WP_011003398.1	GntR family transcriptional regulator	-
HI799_RS19280	68822..69373	-	552	WP_016722343.1	helix-turn-helix transcriptional regulator	-
HI799_RS19285	69506..70105	+	600	WP_016722344.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	cyaB / flgC / flgG / fliM / fliG / fliI / acrB / adeG / cheY / cheB / cheW / motA	1..2075666	2075666
	-	flank	IS/Tn	-	-	62065..63051	986

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (2-67)

Antitoxin

• Cro/C1-type helix-turn-helix domain (52-96)

Sequences

Toxin

Protein

• Length: 76 a.a.
• Molecular weight: 8566.85 Da
• Isoelectric Point: 7.2789

>T155520 WP_016722340.1 NZ_CP052117:c66342-66115 [Ralstonia solanacearum]
VRIDRLQMGHPGNMKAVRDGIRELRIDHGPGDRLYFVRHGVVLIVLLCGGDKSTQETDIRRAIDLSRRLDVDDME

Nucleotide

• Length: 228 bp

>T155520 NZ_CP068805:3143180-3143674 [Escherichia coli]
ATGATTCAGGCCCCCGAGCCACTCATGGCAAGGCATCAATTCACCTCTTTTTGCTCGGGAGTAGAAACGATGGATAACTG
GCTAAAGCAGAGAGCCCTAAAAAATCAGCTTGCCGGCGCATCACGTACATTTGTTAGCTGTGATACGTATAGCAACGTTT
TGGCATATTATTCGCTTGCTTCCAGCGCCGTTGAAACGTATGTCGCGACAGGACGATTTCGCCGAAATATGCCAGAACCG
ATCCCTGTTGTAGTACTTGGTCGTCTGGCAATAGATAAATCATTACAGGGTCAGGGCATCGGTCGTGCGATGGTCAGAGA
TGCCGGTCTACGAGTACTACAGGCGGCTGAAGTCATTGGTATTCGCGGAATGTTGGTCCATGCGTTATCAGACCAGGCCC
GTGAGTTTTATCTGCGAGTGGGGTTTGAACCATCGCCTGTCGATTCTATGATACTGATGGCAACATTGGCTGATTTGCAG
GAATGTCTTAAATAG

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 10967.57 Da
• Isoelectric Point: 7.2780

>AT155520 WP_016722339.1 NZ_CP052117:c66118-65813 [Ralstonia solanacearum]
MTMAQRTIKTRPWDSAEHLRTEADIAAYLDACLEEAGDDPAFITHALGVVARSRGMTQLARETGMTREGLYKALSEGGNP
SFATVLKVIRALGIRLHAVPT

Nucleotide

• Length: 306 bp

>AT155520 NZ_CP068805:3142890-3143183 [Escherichia coli]
ATGCCAGCAGCCAATAACATTCCAACCAGGCGCGAATCTTTAAATTTGCGCATAAAACCCGCAGAACGCGATCTTATTGA
CCGGGCCGCTAAAGCCCGAGGAAAAAATCGCACCGAATTTGTCCTTGAGGCCGCCAGAGCCGCAGCTGAAGAGGCGCTCA
TTGAGCAGAGAATTTTCAGGATCGATTCTGATGATTACCAGCTATTTTTGAATCGTCTGGATCAGCCCCCCGCGCCCAAT
GCTGCATTACGCAAAACCATGCAAACACCCGCGCCGTGGGAGCAAAAAAAATGA