Detailed information of TA system TA155513

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 1965355..1965949
• Replicon: chromosome
• Accession: NZ_CP052114
• Organism: Ralstonia solanacearum strain FJAT1463.F50

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: G3A6N4
• Locus tag: HI800_RS09540
• Protein ID: WP_016721901.1
• Coordinates: 1965686..1965949 (-)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: HI800_RS09535
• Protein ID: WP_038961924.1
• Coordinates: 1965355..1965693 (-)
• Length: 113 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HI800_RS09505	1961737..1962075	+	339	WP_016721894.1	phage tail protein	-
HI800_RS09510	1962072..1962749	+	678	WP_016721895.1	phage tail protein	-
HI800_RS09515	1962755..1963351	+	597	WP_016721896.1	tail fiber assembly protein	-
HI800_RS09520	1963348..1964049	+	702	WP_071895496.1	phage minor tail protein L	-
HI800_RS09525	1964051..1964761	+	711	WP_071895497.1	C40 family peptidase	-
HI800_RS09530	1964765..1965358	+	594	WP_016721899.1	tail assembly protein	-
HI800_RS09535	1965355..1965693	-	339	WP_038961924.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
HI800_RS09540	1965686..1965949	-	264	WP_016721901.1	type II toxin-antitoxin system HicA family toxin	Toxin
HI800_RS09545	1966002..1969157	+	3156	WP_016721902.1	host specificity protein J	-
HI800_RS09550	1969494..1970150	+	657	WP_038961925.1	hypothetical protein	-
HI800_RS09555	1970219..1970710	+	492	WP_038961926.1	glycoside hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1895923..1988942	93019

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-75)

Antitoxin

• Uncharacterised protein family HicB (56-105)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 9834.30 Da
• Isoelectric Point: 8.7458

>T155513 WP_016721901.1 NZ_CP052114:c1965949-1965686 [Ralstonia solanacearum]
MKAKHKKTLDLIFSRPTPAGVKWNDLVGLLVELGAEITEREGSRVAVFLFNQVKVMHRPHPSPDVDKGAVASIRKWLEDN
ATEWNNE

Nucleotide

• Length: 264 bp

>T155513 NZ_CP068805:1990278-1990532 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 113 a.a.
• Molecular weight: 12183.80 Da
• Isoelectric Point: 4.8946

>AT155513 WP_038961924.1 NZ_CP052114:c1965693-1965355 [Ralstonia solanacearum]
MNNVMIIGGEKAVISFDPELEMFRGEFIGLNGGADFYASDVAGLHREGELSLRTFMEECVRRGVEPRRSFSGKFVLRTSE
KTHEAAAIAAAAHGVSLNQWVSDVIEQAAIPA

Nucleotide

• Length: 339 bp

>AT155513 NZ_CP068805:1990030-1990281 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Structures

Toxin

• AlphaFold DB: G3A6N4