Detailed information of TA system TA155480

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/COG3657-dnstrm_HI1420
• Location: 50762..51291
• Replicon: plasmid Plas1
• Accession: NZ_CP052099
• Organism: Ralstonia solanacearum strain FJAT15304.F50

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: HI808_RS17280
• Protein ID: WP_011003396.1
• Coordinates: 51064..51291 (-)
• Length: 76 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A0S4U6E5
• Locus tag: HI808_RS17275
• Protein ID: WP_064478231.1
• Coordinates: 50762..51067 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HI808_RS17245	46264..46749	+	486	WP_016726511.1	hypothetical protein	-
HI808_RS17250	47080..47361	-	282	WP_016722336.1	hypothetical protein	-
HI808_RS17255	47492..47776	-	285	WP_011003391.1	hypothetical protein	-
HI808_RS17260	48109..48296	+	188	Protein_46	IS5/IS1182 family transposase	-
HI808_RS17265	48463..49131	+	669	WP_038938024.1	hypothetical protein	-
HI808_RS17270	49236..50549	-	1314	WP_064478230.1	metabolite/H+ symporter	-
HI808_RS17275	50762..51067	-	306	WP_064478231.1	putative addiction module antidote protein	Antitoxin
HI808_RS17280	51064..51291	-	228	WP_011003396.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HI808_RS17285	51447..52646	-	1200	WP_071623818.1	MFS transporter	-
HI808_RS17290	52773..53594	-	822	WP_011003398.1	GntR family transcriptional regulator	-
HI808_RS17295	53772..54323	-	552	WP_011003399.1	helix-turn-helix transcriptional regulator	-
HI808_RS17300	54456..55055	+	600	WP_064478235.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	cyaB / flgC / flgG / fliM / fliG / fliI / acrB / adeG / cheY / cheB / cheW / motA	1..2053891	2053891
	-	flank	IS/Tn	-	-	45140..46105	965

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-62)

Antitoxin

• Cro/C1-type helix-turn-helix domain (52-96)

Sequences

Toxin

Protein

• Length: 76 a.a.
• Molecular weight: 8544.80 Da
• Isoelectric Point: 6.4584

>T155480 WP_011003396.1 NZ_CP052099:c51291-51064 [Ralstonia solanacearum]
VRIDRLQMGNPGDMKAVRDGIRELRIDHGPGDRLYFVRHGVVLIVLLCGGDKSTQETDIRRAIDLSRRLDVDDME

Nucleotide

• Length: 228 bp

>T155480 NZ_CP052099:c51291-51064 [Ralstonia solanacearum]
GTGCGCATCGACCGCCTGCAGATGGGCAACCCGGGCGACATGAAAGCGGTCCGTGACGGCATCCGTGAGCTGCGGATCGA
TCATGGGCCGGGCGACCGCTTGTACTTCGTGCGGCATGGCGTGGTCCTGATCGTACTGCTCTGCGGCGGCGACAAGTCGA
CGCAGGAAACCGATATCCGTCGCGCAATTGACCTGTCCCGCCGTCTCGACGTGGACGACATGGAATGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 10983.62 Da
• Isoelectric Point: 7.2780

>AT155480 WP_064478231.1 NZ_CP052099:c51067-50762 [Ralstonia solanacearum]
MTMAQRTIKTRLWDSAEHLRTEADIAAYLDACLEEAGDDPAFITHALGVVARSRGMTQLARETGMTREGLYKALSEGGNP
SFATVLKVIRALGIRLHAVPT

Nucleotide

• Length: 306 bp

>AT155480 NZ_CP052099:c51067-50762 [Ralstonia solanacearum]
ATGACGATGGCCCAACGCACCATCAAGACCCGGCTGTGGGATTCGGCCGAACACCTCCGGACCGAAGCGGACATCGCCGC
CTACCTCGACGCCTGCCTGGAAGAAGCCGGAGATGACCCCGCCTTCATCACGCATGCGCTGGGCGTGGTCGCGCGCTCGC
GCGGCATGACGCAGCTCGCCCGCGAGACCGGGATGACCCGCGAGGGGCTGTACAAGGCGCTTTCCGAGGGCGGCAATCCG
AGCTTTGCGACCGTGCTGAAGGTCATCCGGGCGCTCGGCATCCGGCTGCACGCCGTGCCGACCTGA

Structures

Antitoxin

• AlphaFold DB: A0A0S4U6E5