Detailed information of TA system TA155475

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 2070938..2072533
• Replicon: chromosome
• Accession: NZ_CP052096
• Organism: Ralstonia solanacearum strain FJAT15304.F6

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A0S4UDK5
• Locus tag: HI809_RS09405
• Protein ID: WP_069079583.1
• Coordinates: 2071196..2072533 (+)
• Length: 446 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A0S4UDK3
• Locus tag: HI809_RS09400
• Protein ID: WP_016725279.1
• Coordinates: 2070938..2071192 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HI809_RS09375	2067001..2067927	-	927	WP_016727232.1	muramoyltetrapeptide carboxypeptidase	-
HI809_RS09380	2067924..2068475	-	552	WP_016727233.1	tRNA adenosine(34) deaminase TadA	-
HI809_RS09385	2068495..2068971	-	477	WP_011001399.1	6-carboxytetrahydropterin synthase QueD	-
HI809_RS09390	2069039..2069677	-	639	WP_020831956.1	7-carboxy-7-deazaguanine synthase	-
HI809_RS09395	2069995..2070834	+	840	WP_016725278.1	oxaloacetate decarboxylase	-
HI809_RS09400	2070938..2071192	+	255	WP_016725279.1	helix-turn-helix transcriptional regulator	Antitoxin
HI809_RS09405	2071196..2072533	+	1338	WP_069079583.1	type II toxin-antitoxin system HipA family toxin	Toxin
HI809_RS09410	2072530..2073732	+	1203	WP_064047701.1	MFS transporter	-
HI809_RS09415	2073958..2074686	+	729	WP_011001393.1	DsbA family protein	-
HI809_RS09420	2074699..2075262	-	564	WP_080606483.1	RNA 2',3'-cyclic phosphodiesterase	-
HI809_RS09425	2075443..2076141	-	699	WP_016725453.1	protocatechuate 3,4-dioxygenase subunit alpha	-
HI809_RS09430	2076138..2076878	-	741	WP_011001390.1	protocatechuate 3,4-dioxygenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (6-107)

Antitoxin

• Transcription regulator, HipB-like (10-66)

Sequences

Toxin

Protein

• Length: 446 a.a.
• Molecular weight: 48724.75 Da
• Isoelectric Point: 6.7232

>T155475 WP_069079583.1 NZ_CP052096:2071196-2072533 [Ralstonia solanacearum]
MAHPELHLWMNGEHVGIWTASRAGVPILRYDDGWLRSPNMRPLSLSLPIPAGVPELRGQVVDDYFDNLLPEAQPIRERVR
RRFGLASTDAAELLAAIGRDCVGAVQLLPPGMAPTGYDRIDSEPLTDSQVDTLLRNLTATPALGEDAGDPDAFRISIAGA
QEKTALLRIGDAWHRPLGATPTTHILKLPLGLIGNIRADMQDSVDNEWLCAQLLAAWGLEVARTEIARFGASKVLAVERF
DRRWMPGNAWIARLPQEDFCQATGTPAHRKYENDGGPGMRACLDLLSASEHAIDDKRRFVLAQLAFWLLAATDGHAKNFS
LAIGRGGRFRMTPLYDVLSVWPIMGDGPNQLSPYRARLAMALRGKNAHARLADIHVRHWQALAAQSGVPDAFAGMQALVE
RVPAGLERVEAILPPDFSGRVWSSVRTGVLAQAERFLNELASVTP

Nucleotide

• Length: 1338 bp

>T155475 NZ_CP068803:c4345458-4345285 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 8754.07 Da
• Isoelectric Point: 10.4693

>AT155475 WP_016725279.1 NZ_CP052096:2070938-2071192 [Ralstonia solanacearum]
MESLLSSAPQLSAHLKSLRKARGLTQAQLGALLGVSQNRIADIEKDPGAVGFEQLLRILHALGAQLTLRTSAPAGTPSAA
PADW

Nucleotide

• Length: 255 bp

>AT155475 NZ_CP068803:c4345782-4345486 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CTTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: A0A0S4UDK5

Antitoxin

• AlphaFold DB: A0A0S4UDK3