Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-couple_hipB
Location 2226597..2228183 Replicon chromosome
Accession NZ_CP052084
Organism Ralstonia solanacearum strain FJAT15353.F8

Toxin (Protein)


Gene name hipA Uniprot ID A0A0S4V639
Locus tag HI815_RS10195 Protein ID WP_071507716.1
Coordinates 2226846..2228183 (+) Length 446 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A223GLT8
Locus tag HI815_RS10190 Protein ID WP_028853251.1
Coordinates 2226597..2226842 (+) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HI815_RS10165 2222660..2223586 - 927 WP_020831958.1 muramoyltetrapeptide carboxypeptidase -
HI815_RS10170 2223583..2224134 - 552 WP_028860693.1 tRNA adenosine(34) deaminase TadA -
HI815_RS10175 2224154..2224630 - 477 WP_011001399.1 6-carboxytetrahydropterin synthase QueD -
HI815_RS10180 2224698..2225336 - 639 WP_020831956.1 7-carboxy-7-deazaguanine synthase -
HI815_RS10185 2225654..2226493 + 840 WP_011001397.1 oxaloacetate decarboxylase -
HI815_RS10190 2226597..2226842 + 246 WP_028853251.1 helix-turn-helix transcriptional regulator Antitoxin
HI815_RS10195 2226846..2228183 + 1338 WP_071507716.1 type II toxin-antitoxin system HipA family toxin Toxin
HI815_RS10200 2228180..2229382 + 1203 WP_119417555.1 MFS transporter -
HI815_RS10205 2229608..2230336 + 729 WP_119417556.1 DsbA family protein -
HI815_RS10210 2230349..2230912 - 564 WP_082240038.1 RNA 2',3'-cyclic phosphodiesterase -
HI815_RS10215 2231093..2231791 - 699 WP_173941578.1 protocatechuate 3,4-dioxygenase subunit alpha -
HI815_RS10220 2231788..2232528 - 741 WP_011001390.1 protocatechuate 3,4-dioxygenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(5-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(15-63)


Sequences

Toxin        


Download         Length: 446 a.a.        Molecular weight: 48738.78 Da        Isoelectric Point: 6.7232

>T155455 WP_071507716.1 NZ_CP052084:2226846-2228183 [Ralstonia solanacearum]
MAHPELHLWMNGEHVGIWTASRAGVPILRYDDAWLRSPNMRPLSLSLPIPAGVPELRGQVVDDYFDNLLPEAQPIRERVR
RRFGLASTDAAELLAAIGRDCVGAVQLLPPGMAPTGYDRIDSEPLTDSQVDTLLRNLTATPALGEDAGDPDAFRISIAGA
QEKTALLRIGDAWHRPLGATPTTHILKLPLGLIGNIRADMQDSVDNEWLCAQLLAAWGLEVARTEIARFGASKVLAVERF
DRRWMPGNAWIARLPQEDFCQATGTPAHRKYENDGGPGMRACLDLLSASEHAIDDKRRFVLAQLAFWLLAATDGHAKNFS
LAIGRGGRFRMTPLYDVLSVWPIMGDGPNQLSPYRARLAMALRGKNAHARLADIHVRHWQALAAQSGVPDAFAGMQALVE
RVPAGLERVEAILPPDFSGRVWSSVRTGVLAQAERFLNELASVTP

Download         Length: 1338 bp

>T155455 NZ_CP068802:4114507-4114914 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGTCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 8482.80 Da        Isoelectric Point: 10.4693

>AT155455 WP_028853251.1 NZ_CP052084:2226597-2226842 [Ralstonia solanacearum]
MESLLSSAPQLSAHLKSLRKARGLTQAQLGALLGVSQNRIADIEKDPGAVGFEQLLRILHALGAQLTLRTSAPAAAAPAD
W

Download         Length: 246 bp

>AT155455 NZ_CP068802:4114260-4114526 [Escherichia coli]
ATGTACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAGTGTGACGATCCGGACC
TGTTTAACTGGCTGATGAACCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0S4V639

Antitoxin

Source ID Structure
AlphaFold DB A0A223GLT8

References