Detailed information of TA system TA155453

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-MazE
• Location: 527839..528472
• Replicon: chromosome
• Accession: NZ_CP052084
• Organism: Ralstonia solanacearum strain FJAT15353.F8

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HI815_RS02455
• Protein ID: WP_173941463.1
• Coordinates: 528059..528472 (+)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q8XUX7
• Locus tag: HI815_RS02450
• Protein ID: WP_011002949.1
• Coordinates: 527839..528072 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HI815_RS02435	523427..524722	-	1296	WP_011002952.1	MFS transporter	-
HI815_RS02440	524730..525980	-	1251	WP_089190599.1	AGE family epimerase/isomerase	-
HI815_RS02445	526175..527782	+	1608	WP_058908048.1	glucan biosynthesis protein	-
HI815_RS02450	527839..528072	+	234	WP_011002949.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HI815_RS02455	528059..528472	+	414	WP_173941463.1	PIN domain-containing protein	Toxin
HI815_RS02460	528447..529073	-	627	WP_173941464.1	DUF1415 family protein	-
HI815_RS02465	529264..529647	+	384	WP_173941465.1	hypothetical protein	-
HI815_RS02470	529709..531205	-	1497	WP_173941466.1	glycerol kinase GlpK	-
HI815_RS02475	531371..533107	-	1737	WP_173941467.1	ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-116)

Antitoxin

• SpoVT-AbrB domain (5-37)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 15046.37 Da
• Isoelectric Point: 5.5348

>T155453 WP_173941463.1 NZ_CP052084:528059-528472 [Ralstonia solanacearum]
MPATEAFFDSNVVLYLLSADTAKADAAETLLMTGGVVSVQVLNETTHVMRRKLAMPWHAIETVQEAVRAQCRVEPLTLET
HDLGRRIAERYGLSVYDALIVAAALLAGCNVLYSEDMQHGLVIEPHLRIVNPFSARA

Nucleotide

• Length: 414 bp

>T155453 NZ_CP068802:3358089-3358274 [Escherichia coli]
GTGAAAAGTGCAGACGTGATCGCCATCCTGAAGCAGCATGGCTGGGAACATATTCGAACCCGTGGCAGTCGTCATCAGTT
CCGCCACCCTGTTCATCCGGGTCTGGTGACGGTTCCGCACCCTAAAAAAGATATTAAGCCCGGTACGCTGGCGCAAATTG
GGCGTCAGGCTGGTTTAACATTTTAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8790.98 Da
• Isoelectric Point: 8.7287

>AT155453 WP_011002949.1 NZ_CP052084:527839-528072 [Ralstonia solanacearum]
MQVSKWGNSLAIRLPAAVVEALQLHEGDDVEVVIAGERSFGVRRKRAARELFDRIHQYRGKLPADFIFERDQANARD

Nucleotide

• Length: 234 bp

>AT155453 NZ_CP068802:3358291-3358701 [Escherichia coli]
ATGTTTTATCCCGCCTATATCCACAGTGATCCTGACGGCAGCGCCAGCGGTTTTTTCCCTGATGTTCCAGGCTGTTATTT
TGCTGGTGATACGCTGGACTACGCTTTCCAGGATGCGCGAAGTGCACTGGTCGCGCACTTCGAAACCTTATGCGAGATGA
ATGAAGAGTTGCCGCTTCCAGGCTCTGTGGAAACACATCTGGTCCAGCGTGCGCAGGACTTTATTGGCGGGCAATGGTTG
CTGGTGGATATCAATATGAATCAGTTCGAAGGTCGGGCTGAACGTATCAATATTACGATGCCAAAAAGATTACTGAACAA
GATTGATACCTACGTGCGCAATAACCCAGACTATGCCAACAGAAGTGCGTTTCTGGCGGAAGCTGCCCGGCGTGTATTGC
CGGGCGTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A223GR76