Detailed information of TA system TA155452

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/COG3657-dnstrm_HI1420
• Location: 52583..53112
• Replicon: plasmid Plas1
• Accession: NZ_CP052083
• Organism: Ralstonia solanacearum strain FJAT442.F1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: HI816_RS17385
• Protein ID: WP_016722340.1
• Coordinates: 52885..53112 (-)
• Length: 76 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: HI816_RS17380
• Protein ID: WP_016722339.1
• Coordinates: 52583..52888 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HI816_RS17345	47697..47978	-	282	WP_016722336.1	hypothetical protein	-
HI816_RS17350	48109..48393	-	285	WP_043897982.1	hypothetical protein	-
HI816_RS17355	48726..48913	+	188	Protein_44	IS5/IS1182 family transposase	-
HI816_RS17360	49237..49461	+	225	WP_144061921.1	hypothetical protein	-
HI816_RS17365	49958..50266	+	309	WP_043897983.1	hypothetical protein	-
HI816_RS17370	50600..50887	-	288	WP_043898169.1	barstar family protein	-
HI816_RS17375	51057..52370	-	1314	WP_043897984.1	metabolite/H+ symporter	-
HI816_RS17380	52583..52888	-	306	WP_016722339.1	putative addiction module antidote protein	Antitoxin
HI816_RS17385	52885..53112	-	228	WP_016722340.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HI816_RS17390	53267..54466	-	1200	WP_016726513.1	MFS transporter	-
HI816_RS17395	54593..55414	-	822	WP_011003398.1	GntR family transcriptional regulator	-
HI816_RS17400	55592..56143	-	552	WP_011003399.1	helix-turn-helix transcriptional regulator	-
HI816_RS17405	56276..56875	+	600	WP_016722344.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	cyaB / flgC / flgG / fliM / fliG / fliI / acrB / htpB / adeG / cheY / cheB / cheW / motA	1..1981451	1981451

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (2-67)

Antitoxin

• Cro/C1-type helix-turn-helix domain (52-96)

Sequences

Toxin

Protein

• Length: 76 a.a.
• Molecular weight: 8566.85 Da
• Isoelectric Point: 7.2789

>T155452 WP_016722340.1 NZ_CP052083:c53112-52885 [Ralstonia solanacearum]
VRIDRLQMGHPGNMKAVRDGIRELRIDHGPGDRLYFVRHGVVLIVLLCGGDKSTQETDIRRAIDLSRRLDVDDME

Nucleotide

• Length: 228 bp

>T155452 NZ_CP068802:c3227301-3226927 [Escherichia coli]
ATGAAAACATTACCCGATACACTCGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTGCTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 10967.57 Da
• Isoelectric Point: 7.2780

>AT155452 WP_016722339.1 NZ_CP052083:c52888-52583 [Ralstonia solanacearum]
MTMAQRTIKTRPWDSAEHLRTEADIAAYLDACLEEAGDDPAFITHALGVVARSRGMTQLARETGMTREGLYKALSEGGNP
SFATVLKVIRALGIRLHAVPT

Nucleotide

• Length: 306 bp

>AT155452 NZ_CP068802:c3227759-3227391 [Escherichia coli]
GTGTCAGACACACTCTCCGGGACAACGCATCCCGATGACAATCACGACCGTCCCTGGTGGGGACTACCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCCGACCGCGCCGATATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAACGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCCGAAATGAAAAATTAA