Detailed information of TA system TA155445

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 2187468..2189063
• Replicon: chromosome
• Accession: NZ_CP052078
• Organism: Ralstonia solanacearum strain FJAT445.F1

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A9E8CN90
• Locus tag: HI818_RS09935
• Protein ID: WP_064047702.1
• Coordinates: 2187726..2189063 (+)
• Length: 446 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A9E8CGB2
• Locus tag: HI818_RS09930
• Protein ID: WP_064047703.1
• Coordinates: 2187468..2187722 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HI818_RS09905	2183532..2184458	-	927	WP_020831958.1	muramoyltetrapeptide carboxypeptidase	-
HI818_RS09910	2184455..2185006	-	552	WP_028860693.1	tRNA adenosine(34) deaminase TadA	-
HI818_RS09915	2185026..2185502	-	477	WP_011001399.1	6-carboxytetrahydropterin synthase QueD	-
HI818_RS09920	2185570..2186208	-	639	WP_020831956.1	7-carboxy-7-deazaguanine synthase	-
HI818_RS09925	2186526..2187365	+	840	WP_020831955.1	isocitrate lyase/phosphoenolpyruvate mutase family protein	-
HI818_RS09930	2187468..2187722	+	255	WP_064047703.1	helix-turn-helix transcriptional regulator	Antitoxin
HI818_RS09935	2187726..2189063	+	1338	WP_064047702.1	type II toxin-antitoxin system HipA family toxin	Toxin
HI818_RS09940	2189060..2190262	+	1203	WP_064047701.1	MFS transporter	-
HI818_RS09945	2190488..2191216	+	729	WP_020831952.1	DsbA family protein	-
HI818_RS09950	2191229..2191792	-	564	WP_080511083.1	RNA 2',3'-cyclic phosphodiesterase	-
HI818_RS09955	2191973..2192671	-	699	WP_043897706.1	protocatechuate 3,4-dioxygenase subunit alpha	-
HI818_RS09960	2192668..2193408	-	741	WP_011001390.1	protocatechuate 3,4-dioxygenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (5-107)

Antitoxin

• Transcription regulator, HipB-like (10-66)

Sequences

Toxin

Protein

• Length: 446 a.a.
• Molecular weight: 48768.81 Da
• Isoelectric Point: 6.7232

>T155445 WP_064047702.1 NZ_CP052078:2187726-2189063 [Ralstonia solanacearum]
MAHPELHLWMNGEHVGIWTASRAGVPILRYDDAWLRSPNMRPLSLSLPIPAGVPELRGQVVDDYFDNLLPEAQPIRERVR
RRFGLASTDAAELLAAIGRDCVGAVQLLPPGMAPTGYDRIDSEPLTDSQVDTLLRNLTATPALGEDAGDPDAFRISIAGA
QEKTALLRIGDAWHRPLGATPTTHILKLPLGLIGNIRADMQDSVDNEWLCAQLLAAWGLEVARTEIARFGTSKVLAVERF
DRRWMPGNAWIARLPQEDFCQATGTPAHRKYENDGGPGMRACLDLLSASEHAIDDKRRFVLAQLAFWLLAATDGHAKNFS
LAIGRGGRFRMTPLYDVLSVWPIMGDGPNQLSPYRARLAMALRGKNAHARLADIHVRHWQALAAQSGVPDAFAGMQALVE
RVPAGLERVEAILPPDFSGRVWSSVRTGVLAQAERFLNELASVTP

Nucleotide

• Length: 1338 bp

>T155445 NZ_CP068802:1886994-1887212 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 8780.11 Da
• Isoelectric Point: 10.0897

>AT155445 WP_064047703.1 NZ_CP052078:2187468-2187722 [Ralstonia solanacearum]
MESLLSSAPQLSAHLKSLRKARGLTQAQLGALLGVSQNRIADIEKDPGAVGFEQLLRILYALGAQLTLRTSAPAGTPSAA
PADW

Nucleotide

• Length: 255 bp

>AT155445 NZ_CP068802:1886594-1886968 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG