Detailed information of TA system TA155432

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 1925077..1925671
• Replicon: chromosome
• Accession: NZ_CP052072
• Organism: Ralstonia solanacearum strain FJAT448.F50

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: G3A6N4
• Locus tag: HI821_RS09240
• Protein ID: WP_016721901.1
• Coordinates: 1925408..1925671 (-)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: HI821_RS09235
• Protein ID: WP_038961924.1
• Coordinates: 1925077..1925415 (-)
• Length: 113 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HI821_RS09205	1921459..1921797	+	339	WP_016721894.1	phage tail protein	-
HI821_RS09210	1921794..1922471	+	678	WP_016721895.1	phage tail protein	-
HI821_RS09215	1922477..1923073	+	597	WP_016721896.1	tail fiber assembly protein	-
HI821_RS09220	1923070..1923771	+	702	WP_071895496.1	phage minor tail protein L	-
HI821_RS09225	1923773..1924483	+	711	WP_071895497.1	C40 family peptidase	-
HI821_RS09230	1924487..1925080	+	594	WP_016721899.1	tail assembly protein	-
HI821_RS09235	1925077..1925415	-	339	WP_038961924.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
HI821_RS09240	1925408..1925671	-	264	WP_016721901.1	type II toxin-antitoxin system HicA family toxin	Toxin
HI821_RS09245	1925724..1928879	+	3156	WP_016721902.1	host specificity protein J	-
HI821_RS09250	1929216..1929872	+	657	WP_038961925.1	hypothetical protein	-
HI821_RS09255	1929941..1930432	+	492	WP_038961926.1	glycoside hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1855645..1939855	84210

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-75)

Antitoxin

• Uncharacterised protein family HicB (56-105)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 9834.30 Da
• Isoelectric Point: 8.7458

>T155432 WP_016721901.1 NZ_CP052072:c1925671-1925408 [Ralstonia solanacearum]
MKAKHKKTLDLIFSRPTPAGVKWNDLVGLLVELGAEITEREGSRVAVFLFNQVKVMHRPHPSPDVDKGAVASIRKWLEDN
ATEWNNE

Nucleotide

• Length: 264 bp

>T155432 NZ_CP068801:c4654780-4654490 [Escherichia coli]
ATGAACTATGAGCTGGCGTTTGATCCGCGGGCGCTGAAAGAGTGGCAAAAACTGGGCGTGACAGTCCGGGAACAATTTAA
AAAAAAGCTTGAGGATGTGCTGAAAAGGCCGCGCAATCCATCGGCTAAACTGCGGGATTTACCGGACTGCTACAAAATTA
AGCTGCGCACACAGGGATATCGCCTGGTTTATCAGGTTAACGATAAAGAGTTGCTCGTGTTGGTGATAGCTATTGGTAAG
AGAGAGAACTCAGCCGTTTATGAAGACGCGACTAAACGGCTGGATGAGTGA

Antitoxin

Protein

• Length: 113 a.a.
• Molecular weight: 12183.80 Da
• Isoelectric Point: 4.8946

>AT155432 WP_038961924.1 NZ_CP052072:c1925415-1925077 [Ralstonia solanacearum]
MNNVMIIGGEKAVISFDPELEMFRGEFIGLNGGADFYASDVAGLHREGELSLRTFMEECVRRGVEPRRSFSGKFVLRTSE
KTHEAAAIAAAAHGVSLNQWVSDVIEQAAIPA

Nucleotide

• Length: 339 bp

>AT155432 NZ_CP068801:c4655012-4654770 [Escherichia coli]
ATGGCCACACTTAACGTCCGCCTGGATGACAAACTGAAAAATGAGGCTTATGCGGTGCTGGAAAAATTAAATATCACGCC
TACTGAAGCAGTGCGGCTGCTGTTTCAGTACGTTGCGGAAAATGGACGAATGCCGGTAAAAACGCTCACTGTCAGTGATA
GTGAAGAAGCCTTGTTACGCACTGTCAGGGAACGACTGGCCAACCCACAAGAGGCCATTAAGGTCCGACTGGATGAACTA
TGA

Structures

Toxin

• AlphaFold DB: G3A6N4